CROI 2015 Program and Abstracts

Abstract Listing

Poster Abstracts

WEDNESDAY, FEBRUARY 25, 2015 Session P-N5 Poster Session 2:30 pm– 4:00 pm HCV: Epidemiology and Case Detection 662 Hepatitis C and B Testing Among HIV-Infected Individuals in England Sam Lattimore ; Sarah Collins; Celia Penman; Lukasz Cieply; Sema Mandal

Poster Hall

Sentinel Surveillance of Blood-BorneVirusTesting Public Health England, London, United Kingdom

Background: The British HIV Association guidelines for the management of hepatitis viruses in HIV-positive adults, recommend testing for hepatitis, B and C at HIV diagnosis, and annual thereafter. The sentinel surveillance of blood borne viruses collects laboratory data irrespective of test result; providing information on the population undergoing HIV and hepatitis testing at 15 sentinel laboratories. Methods: Demographic information and HBV and HCV testing histories for persons newly diagnosed with HIV between 2008 and 2013 were extracted from the laboratory information systems of 15 sentinel laboratories in England. Service type and age at HIV diagnosis was recorded. Ethnicity was assigned using name analysis software. Duplicate records, reference testing and individuals aged <16 years at HIV diagnosis were excluded. Characteristics of individuals tested and testing positive for HIV, HBV and HCV were described. Data were managed in MS Access and analysed in R (CRAN). Results: In total, 1,936,792 persons were tested for HIV across all settings, of whom 17,962 (0.9%) tested positive. Two thirds of all HIV positive persons were male (12,228/17,691) and where known, three quarters were white ethnic origin (75.9%; 6426/8463). Overall, 75.8% (13,412) of HIV positive persons were tested for anti-HCV, of whom 5.9% (797) tested positive. Of anti-HCV positive persons, 86.3% (688) were tested for HCV-RNA, of whom 75.9% (522) were positive indicating an active infection. HCV genotype information was available for 73.3% (383) of all HCV-RNA positive persons. Genotype 1 infections were the most prevalent (61.8%; 237), followed by genotype 3, genotype 4 and genotype 2, representing 23.7%, 12.0% and 2.5%, respectively. HCV genotype distribution did not vary significantly by gender ( χ 2 =1.64; p=0.57), or by age group, but did vary by ethnic group ( χ 2 =19.84; p=0.02). Overall 72.4% (13,011) of HIV positive persons were tested for HBsAg, of whom 4.5% (591) tested positive, of whom 62% (372) were tested for HBV DNA, of whom 84.9% (315) tested positive. Conclusions: Approximately three-quarters of persons newly diagnosed with HIV were tested for HCV and HBV. Of these 522 and 591 persons were identified as being HIV/HCV and HIV/HBV co-infected, respectively. These findings suggest that while screening rates for HCV and HBV are high, further work is necessary to monitor and improve testing rates, and reduce the significant contribution of HCV and HBV towards hepatic morbidity and mortality in HIV infected individuals. 663 WITHDRAWN 664 HCV 2k/1b Recombinant Form among Hepatitis C–Infected Genotype 2 Patients in Georgia Marika Karchava 1 ; JesperWaldenstrom 2 ; Monica M. Parker 3 ; Renee Hallack 3 ; Lali Sharvadze 1 ; Lana Gatserelia 1 ; Nikoloz Chkhartishvili 1 ; Natia Dvali 1 ; Helen Norder 2 ;TengizTsertsvadze 1 1 Infectious Diseases, AIDS and Clinical Immunology Research Center, Tbilisi, Georgia; 2 Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden; 3 Wadsworth Center, Albany, NY, US Background: The first HCV recombinant form, RF2k/1b was initially described in Russia and has since been identified from patients in Ireland, France, Estonia, Uzbekistan and Cyprus. Many of these patients were originally from the country of Georgia. Full genome sequencing studies revealed that this form shares the structural part of the HCV genome from genotype 2 and the non-structural part from genotype 1. As the routine method for genotype identification in Georgia is by the Versant HCV Genotype v2 kit, which amplifies structural Core and 5’UTR regions, nucleotide sequences in non-structural regions will be missed.Therefore, we conducted a small study to compare two genomic regions of HCV for the purpose of HCV recombinant form identification. Methods: We retrospectively sequenced HCV non-structural region 5B (NS5B) in remnant specimens collected from 72 HCV infected Georgian patients with genotypes previously determined with the Versant HCV Genotype v2 kit as: 32 (44.4%) genotype 1, 21 (29.1%) genotype 2 and 19 (26.3%) genotype 3. Of those patients thirty six patient had underwent PEG/RBN treatment. Results: A portion of the NS5B region was sequenced among all 72 specimens, with concordant non-structural/structural results for most genotype 1 and all genotype 3 specimens. Of 21 genotype 2 specimens, 16 showed similarities to genotype 1b. A phylogenetic tree shows that 15 sequences of discordant sequences formed a clade with RF2k/1b reference sequences chosen from different countries. Remaining discordant sequence was found outside the RF2k/1b sub-branch and formed a cluster within genotype 1b. Our findings reveal that among Georgian patients with HCV genotype 2, 76.1% of viral sequences were similar to genotype1b and phylogenetically distinct from genotype 2. .

Poster Abstracts

414

CROI 2015