Research Masterclass in Radiotherapy Physics 2017

3D Cell Culture

500 µm

A more physiologically relevant model than 2D assays due to the presence of hypoxia, gradients of nutrients and increased cell-cell contact

Cells added to ultra-low attachment plates form a 3D spherical structure (‘spheroid’)

Automated imaging and analysis of spheroid growth following radiation was performed with a Celigo  cytometer

Cell viability was also measured using the Cell Titre Glo  assay (measures ATP production)