Research Masterclass in Radiotherapy Physics 2017

Reduced Growth and Viability of tumour spheroids following microbeam Irradiation.

• A549 Spheroids treated with Microbeam or Broadbeam Irradiation

Day 0 Day 7 Day 14

Control

• Growth measured using Celigo  cytometer

• Viability measured at 14 days post treatment

18Gy

• The predicted response of the spheroids to microbeam irradiation was calculated based on the assumption of no communication between cells.

Dose [Gy]

100

• The actual viability following MB falls below the predicted

BB Survival Predicted MB survival

10

BB survival MB survival

• Cell:cell communication plays a role in MB response in 3D culture

Survival [%]

1

0

5

10

15

20