SPSFAM Heavy Metals ERP Book

AOAC Expert Review Panel for Heavy Metals 

AOAC OMA 2016.04: Four Arsenic Species in Fruit Juice, High‐Performance Liquid Chromatography‐ Inductively Coupled Plasma‐Mass Spectrometry, First Action 2016 (ARS‐02)

Was the method's applicability considered?

ER 1 ER 2 ER 3 ER 4 ER 5 ER 6 ER 7 ER 8

Yes Yes Yes Yes Yes Yes Yes Yes

Please state the method's applicability statement:

ER 1 The method describes a procedure for using HPLC in combination with ICP‐MS to determine  inorganic arsenic {iAs, the sum of arsenite [As(UJ)] and arsenate [As(V)]} in clear (free of solids)  fruit juice and fruit juice concentrates (1). Due to difficulties controlling As(lll) and As(V)  interconversion, these  compounds are not repot1ed individually, only as iAs. Dimethylarsinic  acid (DMA) and monomethylarsonic acid (MMA) are also determined with this method. ER 2 Addition of arsenocholine (AsC) to be used as a substitute for AsB was included in the final  draft.  As AsC is significantly cheaper than AsB, the applicability is enhanced.  ER 3 Method is for quantitation of inorganic arsenic (iAs), monomethylarsonic acid (MMA), and  dimethylarsinic acid (DMA) in fruit juice and fruit juice concentrate. ER 4 Quantitation of Arsenic Species in and/or total inorganic arsenic in Selected food and beverage  matrixes. A table presented for selected foods in SMPR 2015.006Comment: the method is  applicable to sperate four species of Aresnic which are Arsenite, Arsenate, Dimethlylarsenic acid  and monomethylarsonic acid but, but they prefer to present the sum of Arsenite and Arsonate  as primary toxic forms by converting Arsenite to Arsenate.  

yes, this method is applicable to fruit juices as part of the SMPR guidance. The method is capable to measure total inorganic Arsenic species (iAs) and also DMA and MMA

ER 5 ER 6

As species in fruit juice

ER 7 To determine inorganic arsenic {iAs, the sum of arsenite [As(III)] and arsenate [As(V)]} in clear  (free of solids) fruit juice and fruit juice concentrates (1). Due to difficulties controlling As(III)  and As(V) interconversion, these compounds are not reported individually, only as iAs.  Dimethylarsinic acid (DMA) and monomethylarsonic acid (MMA) are also determined with this  method ER 8 The method describes a procedure for using HPLC in combination with ICPMS to determine  inorganic arsenic species in fruit juice and fruit juice concentrates.

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