Biophysical Society 59th Annual Meeting Program Guide

12:30 pm –2:00 pm TA Instruments Technology Advances in Ultrasensitive Isothermal Titration Calorimetry TA Instruments introduces the Affinity ITC, with all new technology for advanced isothermal titration calorimetry. Isothermal Titration Calorimetry is the most effective analytical tool for simply and accurately measuring A/B interactions, especially protein-protein binding. Isothermal Titration Calorimetry provides complete thermodynamics and kinetics without label- ling, fixing, or otherwise altering the sample of interest. All new technology from TA Instruments improves the sample throughput, usability, and data quality of all isothermal titration calorimetry experiments. All-new advanced stirring technology and an innovative isolated injection system improves baseline stability, and mixing homogeneity while applying minimal perturbation to the material of interest. For large-scale screening and high throughput testing, an all-new unattended sample handling sys- tem automates up to 96 full titrations and continuous unattended opera- tion for multiple days. Based around a 96-well plate format and multiple wash/rinse containers, the Affinity ITC Auto will greatly increase laboratory productivity without sacrificing sensitivity or reproducibility. The Affinity ITC is available in both the standard (1.0 mL) and low volume (190 µL) cell sizes, extending the range of applications for which automation is available. This presentation will include data examples and tech tips on experimental design using the Affinity ITC Auto. Presenter Dile Holton, Microcalorimetry Product Manager, TA Instruments 2:30 pm –4:00 pm Bruker Nano Surfaces Super-Resolution Microscopy and Its Applications in Fast and Complex Biological Systems Super-resolution microscopy has revolutionized the field of biological imaging by providing new insights into biological processes in fields as diverse as developmental biology, neuroscience, cardiovascular research, genetics, infectious disease, and DNA/chromatin structure. The Vutara 350 super-resolution microscope offers a ten-fold improvement in resolution in comparison to traditional light microscopy techniques and is capable of achieving resolutions of 20 nm laterally and 50 nm axially. The Vutara 350 is based on a patented 3D biplane single molecule localization platform. We will discuss the basic principles of operation and features of the Vutara 350 super-resolution microscope. The capability to do 3D multicolor imaging, high speed live cell imaging, 3D particle tracking, and z-stacking in various biological systems such as cells, tissue, drosophila, C. elegans, bacteria and virus makes the Vutara 350 very versatile. Presenter Jeff Stuckey, Product Marketing Manager, Bruker Nano Surfaces

4:30 pm –6:00 pm OriginLab Corporation Data Analysis and Graphing Using Origin 2015

Origin is an easy-to-use software application with data analysis and publica- tion-quality graphing for science and engineering. This workshop will cover key features including importing data frommultiple sources including Excel and third-party file formats, LabVIEW connectivity, creating and custom- izing multi-panel graphs, graphical exploration and analysis, curve fitting, peak analysis, signal processing, and statistics. Time saving features such as templates for graphing and analysis, batch plotting and batch analysis will be presented. Application examples using Origin's programming environ- ment will also be presented. The workshop will also cover key new features and improvements in the latest version: Ease-of-use features including graph preview and comment tool tip in Project Explorer, search for string in project, search for functions in dialogs, redesigned axis dialog, enhanced legend, and custom categorical order. New graph types including Heat Map, Kernel Density Plot, Column Scatter Plot. Improvements to profile plot, box plot, contour plot, bubble scale, and color scale. New analysis tools for Distribution Fit, ANOVA of unbal- anced data, t-Test on rows. Tool to append worksheets, remove or combine duplicate values, and improved pivot table. Integration of Python as script- ing language in Origin. Presenter Easwar R. Iyer, VP of Technology, OriginLab Corporation Room B: Monday, February 9 Correlative light and electron microscopy (CLEM) is a powerful approach that enables combining dynamic information and labelling specificity from fluorescence microscopy with ultra-structural information at nano- meter resolution from electron microscopy on the same sample. In recent years technical improvements in fluorescence microscopy have enhanced z-resolution, enabled imaging with high sensitivity using TIRF and, with super-resolution microscopy, improved the resolution of light microscopy to up to 20 nm. Despite all these advances, fluorescence microscopy can only show what was labelled and an EM is needed to provide the full morphological context on the ultra-structure of the cell. However, CLEM experiments still remain challenging and low through-put. Over the last years, FEI has introduced different solutions to overcome some of the challenges in CLEM experiments and to make CLEM experiments easier and more efficient. But correlative experiments are rapidly evolving – here, we will present updates on latest developments that have pushed the boundaries of correlative experiments. Presenter Meike Pedersen , Product Application Specialist, FEI Company 8:30 am –10:00 am FEI Company Advances in Correlative Light and Electron Microscopy

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Biophysical Society 59 th Annual Meeting, Baltimore, Maryland