Biophysical Society 59th Annual Meeting Program Guide

10:30 am –12:00 noon Molecular Devices LLC

a versatile tool for safety and toxicity screening applications serving as an excellent complement to APC. These three platforms enable you to keep up with the requirements of the CiPA-initiative for early prediction of potential cardiac arrhythmias. During this workshop, we will show how to push the boundaries of ion channel screening projects to achieve HTS-screening standards, and how to get ready for comprehensive safety screening beyond hERG. Spaces are limited so reserve yours by sending an email to info@nanion.de. Presenters Niels Fertig, CEO, Nanion Technologies GmbH Andrea Brüggemann, CSO, Nanion Technologies GmbH For over 40 years, Sutter Instrument has designed and produced electro- mechanical and optical instrumentation that helps scientists push the limits. While Sutter has long been the market leader in products for micropipette fabrication and micromanipulation, we have continued to expand our Lambda imaging product line and XenoWorks microinjection systems. A strong emphasis has always been placed on providing expert tech support to help our customers achieve the best results in their research. To further this goal, Sutter Instrument is starting a series of user meetings with tutorial presentations. We will be providing step-by-step guidance to the new experimenter as well as advanced tips and tricks for the experienced user. To round it off, newly introduced products will be discussed on a case-by-case basis. Registration is available online through the Sutter Event Registration Page (http://sutter.eventbrite.com), or by email to info@sutter.com. The number of available spaces is limited, and registrations are accepted on a first-come-first-served basis. Who should attend? • Electrophysiologists who use micropipettes and micromanipulators for patch clamp, sharp electrode or extracellular recordings. • Researchers who perform microinjections, including nuclear transfer, sperm injection and application of substances into cell cultures or intact organisms. • Scientists who want to learn more about optimizing their results with pipette pullers and micromanipulators Presenters Jan Dolzer, Tech Support and Product Development, Sutter Instrument: Introductory Remarks Adair Oesterle, Tech Support Micropipette Fabrication and Microinjection, Sutter Instrument: Optimizing Settings on Your Sutter Micropipette Puller Ali Mahloudji, Tech Support Micromanipulators and Lambda DG Series, Sutter Instrument: Maximizing the Versatility of Your Dual-manipulator Setup 2:30 pm –4:00 pm Sutter Instrument Scientists Empowering Scientists

Performing Positive Allosteric Modulator (PAM) Assays and Investigating Use-Dependent Inhibition of Ion Channels on Automated Electrophysiology Systems Including the IonFlux tm Benchtop Reader and the IonWorks Barracuda® Instrument PAM Assays Nicotinic acetylcholine receptors (nAChRs) have been extensively studied due to their importance in physiological processes as well as involvement in several muscle and neuronal human pathologies, and are major therapeutic targets for pharmaceutical drug discovery. Ensemble recordings on the IonFlux HT System were validated with human hnAChR recombinant cell lines developed by Eurofins Discovery Services. Response properties of the nAChRs to the endogenous ligand acetylcholine (ACh), reference agonists, antagonists and positive allosteric modulators (PAMs) were characterized and will be presented. Ion Channel Use-Dependence Use-dependent inhibition of ion channels by potential drug candidates is an important aspect to investigate for many drug classes. Data will be pre- sented to demonstrate the ability of automated electrophysiology systems to study the use-dependence block of Na+ channel targets by peptide toxins and known compounds. We will demonstrate the ability of the IonWorks Barracuda system to deliver complex voltage protocols and generate long assay windows which are required for these studies.Pulse trains delivered at 10Hz are used to measure the blockade of current. These experiments demonstrate stable assay windows with uniform currents for 30 minutes and longer during the delivery of periodic pulse trains. Presenter James Costantin, Product Marketing Manager, Automated Electrophysiology, Molecular Devices LLC 12:30 pm –2:00 pm Nanion Technologies GmbH HTS-Compatible Giga-Seal Ion Channel Drug Discovery: Beyond the Bottleneck and Ready for CiPA Nanion Technologies is one of the leading providers of automated patch clamp systems, offering a diverse product portfolio covering a broad experimental range from single channel recordings to HTS-compatible ion channel screening from up to 768 cells in parallel. Allowing 20,000 data points per day, the SyncroPatch 384/768PE is unrivalled for high throughput and high quality recordings. Diverse ion channel targets and cell types have successfully been tested on the SyncroPatch 384/768PE including challenging targets such as fast desensitizing ligand ion chan- nels (P2X3 und GluA2), ion channels requiring intracellular activation (Katp, TMEM16a) and heavily regulated channels such as TRPA1. Early cardiac arrhythmic risk assessment is a hot topic these days calling for new safety screening strategies. Patchliner, a medium-throughput APC platform, supports automated current clamp recordings, experiments at physiological temperatures, and a minimal cell usage, making it the ideal partner for safety testing on stem cell derived cardiomyocytes. Additionally, the CardioExcyte 96, a unique hybrid system for parallel impedance-based and MEA-like recordings from intact cardiomyocyte networks, has proven

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Biophysical Society 59 th Annual Meeting, Baltimore, Maryland