Biophysical Society 59th Annual Meeting Program Guide

12:30 pm –2:00 pm Nanion Technologies GmbH Measure More Membrane: Cells, Bilayers and Transporter Activity The Port-a-Patch turned 10 years old last year, and is going stronger than ever. It’s still the smallest patch clamp rig in the world, and makes patch clamp recordings accessible to anyone spending a couple of hours with it. Giga-seal recordings and the excellent voltage-clamp of the cellular membrane ensure high quality data, and the Port-a-Patch add-ons allow unprecedented experimental freedom, including temperature control, inter- nal perfusion, automated action potential recordings, and recordings from primary and stem cell-derived cells. The Orbit 16 is a parallel device for efficient formation of and recordings from up to 16 artificial bilayers at once, for parallel bilayer-reconstitution of ion channels and nanopores. Using Micro Electrode Cavity Array (MECA, Ionera), a 4 x 4 array of circular micro-cavities in a highly inert polymer, the bilayer is automatically formed by remotely actuated painting (Ionera- SPREAD), which all will be demonstrated during the session. Ion transporters and pumps play an important role within general metabo- lism and information processing of organisms. The SURFE2R is a unique platform for direct measurements ion transporters and ion channels in diverse and heterologous membranes. It is easy-to-handle, highly sensitive and a very efficient screening platform. The SURFE2R N1 is a small foot- print, fully automated device recording from membrane preparations, with proven success using native tissue, mammalian and insect cell lines, bacteria, organelles, and proteoliposomes. Join this workshop for hands-on experiments and information about three outstanding platforms: Port-a-Patch, Orbit 16 and SURFE2R N1! We look forward to seeing you! Spaces are limited so reserve yours by sending an email to info@nanion.de. Presenters

4:30 pm –6:00 pm Molecular Devices LLC Eliminating 50-60 hz Line-frequency Noise with the New HumSilencer and pCLAMP Software Tips &Tricks

We will introduce a new feature of the Axon DigidataTM 1550A digitizer, HumSilencer, which provides a smart and simple method for eliminating 50 or 60 Hz line-frequency noise. In addition, we will present solutions to frequently asked questions on our pCLAMP software, a powerful data acquisition and analysis software that is used widely for a variety of electro- physiological recordings in many academic laboratories. Presenter Jeffrey Tang, Axon Product Marketing Manager, Molecular Devices LLC Room B: Tuesday, February 10 Learn How SensiQ’s Dynamic Injection (diSPR®) Techniques Enhance the Biophysical Characterization of Binding Events Using Surface Plasmon Resonance Technology SensiQ’s dynamic injection methods provide complete, one-pass kinetic and equilibrium data from a single injection while reducing statistical error/ noise. Simply load one, highest analyte concentration vial and the instru- ment exposes the surface to either a stepwise (FastStep®) or continuous gradient (OneStep®) of concentrations. These approaches increase the ease/ throughput of SPR experiments and provide complete data sets for interac- tions that are complicated by incomplete surface regeneration. FastStep® uses a patented onboard micro-mixing technique to create increas- ing fixed concentrations of analyte in real time without generating partial dissociation responses as the instrument prepares subsequent concentra- tions. This technique improves throughput by decreasing the time to com- plete a full run while simplifying data analysis. OneStep® is the ultimate evolution of FastStep®. Taylor dispersion fluidics establish a continuous gradient of analyte concentrations which is flowed over the surface to generate a sigmoidal binding curve. This technique introduces a time dependent variable that is not possible in traditional injection techniques and allows for the quantitative separation of multiple binding sites with different affinities. OneStep® also increases the dynamic range of allowable concentrations thereby removing the need to perform test injections or accurately guess the affinity of an unknown interaction. Importantly, OneStep® also provides added data content in SPR experi- ments by providing a measure of the analyte diffusion coefficient to help identify analytes that have a tendency to oligomerize or aggregate. SensiQs’ operational software was developed to simplify assay development and instrument operation. Using a drag and drop icon based program- ming approach, traditional program “scripting” is eliminated to simplify and speed assay development. Executable protocols for high throughput experi- ments can be developed in minutes. Programming examples will show how operational actions have been optimized to decrease runtime and increase throughput. Streamlining data analysis of small or large data sets using our Q-Dat software will also be presented. Presenters Derek Beahm, SensiQ Application Scientist Rick Cope, SensiQ Sales Representative 10:30 am –12:00 noon SensiQ Technologies Inc

Andrea Brüggemann, CSO, Nanion Technologies GmbH Maria Barthmes, Application Specialist, Nanion Technologies Gerhard Baaken, CEO, Ionera

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Biophysical Society 59 th Annual Meeting, Baltimore, Maryland