SPSFAM Allergens ERP

2. Does the method contain system suitability tests or controls as specified by the SMPR? If not, please indicate if there is a need for such tests or controls and which ones. 3. Is there information demonstrating that the method system suitability tests and controls as specified in the SMPR worked appropriately and as expected? If no, please specify. 4. Based on the supporting information, is the method written clearly and concisely? If no, please specify the needed revisions.

Yes

It is not well detailed.

The method is clearly written but there are several information missing.

The proper allergen material (whole allergenic food and roasted peanuts and hazelnuts) to be used

Recovery studies

Use of QC points

Describe how LOD was calculated and provide a criteria for establishment of MDL (use of a specific quantifier peptide?

The total analysis time is now specified

5. Based on the supporting information, what are the pros/strengths of the method? 6. Based on the supporting information, what are the cons/weaknesses of the method?

Strengths: The method could be able to monitor traces of all nuts in different food commodities also including soy that might be an added value to method extension.

Weaknesses: The LODs and LOQs reflect a different type of spike reralized in the food therefore the LODs achieved are not in agreement with the perormance requirements detailed in the SMPR document. The calculation of the recovery is also not in line with what expected.

The protocol is not properly described and experimental steps not well detailed. It is not clear which was the most sensitive peptide found.

7. Any general comments about the method?

There are several parts that should be improved and some part of the work carried out.

Do you recommend this method be adopted as a First Action and published in the Official Methods of Analysis of AOAC INTERNATIONAL? Please specify rationale.

No; there several experimental details missing and points along the method that must be improved to meet requirements of SMPR.