AOAC OMB Final Action Recommendation (December 2019)-2016.14

2016.14 (Jan. 2019) FOS-03 MLT Report FOR ERP USE ONLY DO NOT DISTRIBUTE

Table 10. Flow Gradient used by lab 7

Flow rate, mL/min

NaOH, mM

NaOAc, mM

Time, min

0.0

0.4 0.4 0.5 0.5 0.8 0.8

200 200 200 200 200 200 200 200

2 2 2 2 2 2 2 2

20.0 20.1 26.0 26.1 56.0 56.1 60.0

0.4 0.4

Table 11. Waveform for flexIPAD detection used by lab 7

Duration, ms

Sum duration, ms Start potential,V

End potential, V

370 150 110 120

370 520 630 750 800 840 900

0.0 0.0

0.0

0.28 0.28

0.28 0.28

0.0 0.0

50 40 60

0.0

-1.0

-1.0

0.6

0.6

b) Child formula powder

a) Standard solution Level 4

Figure 4. Example of chromatograms obtained by lab 7, using Metrosep Carb 2 column without post- column addition of NaOH and flow gradient described in Table 10