AOAC OMB Final Action Recommendation (December 2019)-2016.14

FOS-03 FOR WORKING GROUP/ERP USE ONLY DO NOT DISTRIBUTE

b s VV Vm a s



C



1000 

s



Where:

Cs = Concentration of fructose standard solution (in μg/mL)

m s = Weight of fructose used to make the fructose stock solution (in mg), normally about 100 mg

V a = Volume of fructose stock solution used to prepare the fructose standard solution (in mL), normally about 0.7 mL

V b = Final volume of the fructose stock solution (in mL), normally 100 mL

V s = final volume of the fructose standard solution (in mL), normally 100 mL

1000 = factor to convert mg/mL into μg/mL

The correction factors k and p can be calculated from the ingredient DP as follows:

DP

180

162 (

   ))1 (

k



DP

180



DP

p



DP

1 

Where:

DP = average degree of polymerisation of the fructan (i.e. the average number of

monosaccharide residues per chain).

k = correction factor for the uptake of water during hydrolysis.

p = correction factor to correct for loss of terminal fructose in F m

type fructans or terminal

glucose in GF n

type fructans.

The concentration of fructan in the product can be calculated as follows:

CVA s

3.01.1    

1

1

C

p k



100 

1 mA

4.02.0   

s