Vi-CELL BLU -Application Notes

Cell Count Results A dilution protocol of 8 serial dilutions of CHO cells was established to test cell counting performance over different concentration ranges. The lower range concentrations were 3 replicate plates were run on 3 Vi-CELL BLU instruments. Higher concentration ranges were run as 3 sets of replicate samples of 10 tubes per dilution using the carousel due to limited sample availability.

Dilution

Nominal Concentration (x10^6) cells/mL

100%

5.50

80%

4.40

60%

3.30

50%

2.75

40%

2.20

30%

1.65

20%

1.10

10%

0.55

Average of Concentration (x10^6 cells/mL

%CV of Total (x10^6) cells/mL

Average of Viability (%)

Average of Cell count

%CV of Viability (%)

%CV of Cell count

# Samples

Instrument Dilutions (n=12)

100%

14524

2.51%

5.47

2.50%

63.97

1.69%

12

80%

11099

2.79%

4.18

2.78%

64.77

0.70%

12

60%

8528

4.15%

3.21

4.10%

64.39

1.28%

12

50%

6848

2.81%

2.58

2.72%

64.33

1.20%

12

B01

40%

5795

9.84%

2.18

9.83%

61.93

2.22%

12

30%

3960

2.93%

1.49

2.82%

62.07

1.33%

12

20%

2617

4.71%

0.99

4.63%

60.88

2.10%

12

10%

1185

4.25%

0.45

4.14%

58.41

3.49%

12

100%

14736

3.44%

5.52

3.43%

65.81

1.17%

12

80%

11535

3.62%

4.32

3.60%

64.58

0.79%

12

60%

8479

2.27%

3.17

2.27%

65.79

1.15%

12

50%

7087

3.94%

2.65

3.92%

64.50

1.36%

12

B02

40%

5547

3.31%

2.08

3.32%

62.36

1.22%

12

30%

4234

7.24%

1.59

7.19%

64.33

1.68%

12

20%

2820

6.27%

1.06

6.33%

61.88

1.87%

12

10%

1250

6.55%

0.47

6.42%

58.28

3.70%

12

100%

14999

2.24%

5.42

2.34%

65.50

1.16%

12

80%

11834

2.39%

4.27

2.40%

64.80

0.94%

12

60%

8776

1.91%

3.17

1.88%

65.51

0.91%

12

50%

7281

2.78%

2.63

2.79%

64.40

1.48%

12

B03

40%

5658

3.12%

2.04

3.11%

61.48

1.13%

12

30%

4138

4.71%

1.49

4.74%

62.58

2.08%

12

20%

2644

4.10%

0.96

3.93%

60.79

1.91%

12

10%

1237

4.25%

0.45

4.14%

58.36

3.81%

12

Characterized by Ingenuity | 4