Vi-CELL BLU -Application Notes

Results

Cell Concentrations for 8 serial dilutions of CHO cells Low Concentration ~0.5M -5M Range. 36 samples per dilution

6.00

R = 0.9996

5.00

4.00

3.00

Cell Concentration (M/mL)

2.00

1.00

0.00

0%

10%

20%

30%

40%

50%

60%

70%

80%

90%

100%

Dilution %

Figure 3. CHO Cells Low Concentration Range Dilution Series Data

Cell Concentrations for 7 serial dilutions of CHO cells High Concentration ~2.6M-13M Range. 30 samples per dilution

14.00

R = 0.9988

12.00

10.00

8.00

6.00

Cell Concentration (M/mL)

4.00

2.00

0.00

0%

10%

20%

30%

40%

50%

60%

70%

80%

90%

100%

Dilution (%)

Figure 4. CHO Cells High Concentration Range Data

Additional sample plates were run using a smaller range of dilutions to confirm the performance of the instruments. These were repeated 3 times within a 16 hour period using the same stock supply of cells run on the same instrument. During this period cell population increase is considered minimal and the source material for analysis effectively the same.

Characterized by Ingenuity | 4