Vi-CELL BLU -Application Notes

1

2

3

4

5

6

7

8

9 10 11

12

A

MT01

B

MT02 MT03 MT04 MT05 MT06

C

D E F G

MT07

Run Orientation

H

MT08

Figure 1. Plate layout for cell type analysis

Results The results from the run were imported into Microsoft Excel™ and a pivot table used to extract the data. The results were plotted as simple High:Low charts to determine the effects of the different cell type settings on the concentration, viability and average diameter values.

Effectof MaxDiameter onViability

Effectof MaxDiameter onAverageDiameter

Effectof MaxDiameter onCell Concentra�on

14.00%

9.00%

9.00%

L07 L01 L02

L07 L01 L02

L07 L01 L02

9.00%

4.00%

4.00%

4.00%

-1.00%

-1.00%

20

40

20

40

-1.00%

20

40

-6.00%

-6.00%

-6.00%

-11.00%

-11.00%

-11.00%

Effectof VSBon Viability %

Effectof VSBon on AverageDiameter

Effectof VSBon Cell Concentra�on

10.00%

10.00%

10.00%

L07 L01 L02

L07 L01 L02

L07 L01 L02

0.00%

0.00%

0.00%

40

90

40

90

40

90

-10.00%

-10.00%

-10.00%

Effectof DeclusteronViability %

Effectof Declusteronon AverageDiameter

Effectof DeclusteronCell Concentra�on

17.50%

17.50%

17.50%

12.50%

12.50%

12.50%

L07 L01 L02

L07 L01 L02

L07 L01 L02

7.50%

7.50%

7.50%

2.50%

2.50%

2.50%

High

Low

High

Low

-2.50%

-2.50%

High

Low

-2.50%

-7.50%

-7.50%

-7.50%

-12.50%

-12.50%

-12.50%

-17.50%

-17.50%

-17.50%

Figure 2. High:Low plots for adjusted cell type parameters.

The results show that maximum diameter and decluster degree have significant effects on cell concentration whereas little effect on viability percentage. Viable spot brightness has the most significant impact on viability values as may be expected. The above plots can help establish our expectations of what will happen when we adjust these particular parameters. However if our goal is to match Vi-CELL XR values this approach can assist tremendously if we also run the same samples as above on the Vi-CELL XR at the same time as the above data is being collected. Then we can obtain comparison data on the same cell samples.. This was conducted for an experiment running 10 samples on 3 different Vi-CELL XRs with the default CHO cell type for the analysis. The results below show the different cell type results from the Vi-CELL BLU machines (columns) overlaid with the average results from the Vi-CELL XR instruments (lines).

Characterized by Ingenuity | 2