AOAC Annual Meeting_AIMS

Negative Process control – A known negative sample run through the extraction process and then tested by PCR to provide evidence that large scale cross contamination did not occur. Positive Process control – A sample known to be positive run through the extraction process and then tested by PCR to demonstrate that all phases of the method were preformed successfully. Surrogate Organism – A non- Cyclospora cayetanensis protozoan parasite used as an indicator that the extraction and detection method worked successfully. It can also be used as a tool to validate a method. Consideration should be given if this organism is genetically or physiologically similar to C. cayetanensis .