AOAC RI ERP E-Book - DS DF

OMAMAN-38 B: Collaborative Study Protocol Expert Review Panel Use Only September, 2017

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Prosky. L, Asp, N.-G., Furda, I., DeVries, J. W., Schweizer, T. F., & Harland, B. F., (1985) J. AOAC Int . 68 , 677-679. McCleary, B. V., McNally, M. & Rossiter, P. (2002). J. AOAC Int . 85, 1103-1111.

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Wehling, P. and DeVries, J. (2012) J. AOAC Int . 95, 1541-1546.

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Tables Table I. Study data for the pre-collaborative evaluation of AOAC Method 2009.01 for total dietary fiber. Table II. Comparable AOAC International method data a . Table III. Interlaboratory study results for total dietary fiber in foods (RINTDF Method). Table IV. Interlaboratory study results for total dietary fiber in foods (RINTDF Method). Statistical evaluation according to AOAC International statistics format a . Figures Figure 2007.xxA. Rapid integrated total dietary fiber assay procedure showing the key steps in the procedure. Figure 2007.xxB. Incubation of samples in Fisherbrand ® incubation bottles in a shaking water bath, showing custom made polypropylene bottle holder. Figure 2007.xxC. 2mag Mixdrive 15 ® submersible magnetic stirrer in custom built bath. With Fisherbrand ® incubation bottles. Figure 2007.xxD. Chromatograms of a mixture of maltodextrins, glucose and glycerol on two TSK gel filtration columns (G2500PWXL) in series. Solvent: distilled water; flow rate: 0.5 mL/min; temperature: 80°C. The arrows show demarcation between DP2 (maltose) and DP 3 (higher maltodextrins). The fraction shown as SDFS denotes the fraction that would be collected as SDFS, however, in this case these are maltodextrins that would be hydrolysed by the PAA/AMG mixture. Figure 2007.xxE. Chromatograms on TSK-Gel G2500PWXL columns of glucose/glycerol mixtures. A mixture of glycerol (100 mg) and glucose (100 mg) was analysed according to the RINTDF procedure. The ethanolic filtrate (for SDFS determination) was concentrated to dryness and re-dissolved in 32 mL of deionised water. A sample of this was analysed by HPLC; a) directly with no desalting and no Bio-Rad ® de-ashing pre-cartridges in place; b) a sample (5 mL) was desalted by AOAC Research I stitute ERP Use Only

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