AOAC RI ERP E-Book - DS DF

K-RINTDF.2016_11_K-RSTAR.DATA.NEW 27/09/2016 11:54 Page 19

OMAMAN-38 C: Instructions for Use Expert Review Panel Use Only September, 2017

HMWDF, IDF or SDFP (mg/100 g)

R 1

+ R 2 - P B - P A - B 2

=

X 100

+ M 2 2

M 1

where:

R 1 = residue mass 1 from M 1 in mg. R 2 = residue mass 2 from M 2 in mg. M 1 = test portion mass 1 in g; M 2 = test portion mass 2 in g. P A = ash mass from R 1 in mg. P B = protein mass from R 2 in mg.

HMWDF (%) = HMWDF (mg/100 g)/1000 IDF (%) = IDF (mg/100 g)/1000 SDFP (%) = SDFP (mg/100 g)/1000

K. CALCULATIONS for SDFS:

SDFS (mg/100 g) = R f x (Wt-IS, mg) x (PA-SDFS)/(PA-IS) x 100/M

where: R f is the response factor (see page 17).

Wt-IS is weight in mg of internal standard (Gly) contained in 1 mL of internal standard solution pipetted into sample mixture (i.e. 100 mg). AOAC Research Institute ERP Use Only PA-SDFS is the peak area of the SDFS. PA-IS is the peak area of the internal standard (glycerol; Gly). M is the test portion mass (M 1 or M 2 ) in grams of the sample whose filtrate was concentrated and analyzed by LC.

L. CALCULATION of TDF:

TDF (%) = HMWDF (%) + SDFS (%)

NOTE: These calculations can be simplified by using the Megazyme Mega-Calc TM , downloadable from where the product appears in the Megazyme web site (www.megazyme.com).

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