AOAC RI ERP E-Book - DS DF

K-RINTDF.2016_11_K-RSTAR.DATA.NEW 27/09/2016 11:54 Page 22

OMAMAN-38 C: Instructions for Use Expert Review Panel Use Only September, 2017

Figure 9. 2mag Mixdrive 15 ® submersible magnetic stirrer in a custom made water bath. This allows stirring of 15 samples at controlled speed (170 rpm) and 37°C.

Figure 10. Grant ® OLS 200 ® shaking incubation bath set at 37°C showing custom made polypropylene holder

for Fisherbrand ® 250 mL bottles.

AOAC Research Institute ERP Use Only

Figure 11. Desalting of samples for HPLC. Five mL of concentrated eluate mixed with 1.5 g of Amberlite ® FPA53 (OH - ) and 1.5 g of Ambersep ® 200 (H + ) resins. Figure 12. Chromatograms on TSK-Gel G2500PWXL columns of a) a non-desalted mixture of glucose and glycerol; b) the same sample after desalting 5 mL with 1.5 g of Amberlite ® FPA53 (OH - ) and 1.5 g of Ambersep ® 200 (H + ) resins (as shown in Figure 11); and c) sample b chromatographed on the same TSK columns, but with a deashing pre-column in place 21 .

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