AOAC RI ERP E-Book - DS DF

OMAMAN-38 E: Safety Checklist & Information Expert Review Panel Use Only September, 2017

11 th May, 2017

Safety Statement Interlaboratory study of the Rapid Intedrated TDF procedure (AOAC Method 2017.xx

Organic solvents: Ethanol or industrial methylated spirits are used to precipitate the high molecular weight fiber components. This solvent should be used in a well ventilated room. Fibre residues are washed with small volumes of acetone (2 x 15 mL). This should be performed in a fume cupboard. Toxic chemicals: In the original Integrated TDF procedure, sodium azide at 0.02% is included in the buffer to prevent microbial contamination over the extended incubation periods (16 h). In the Rapid Integrated TDF procedure, sodium azide has been deleted from buffers. Incubation times are reduced to 4 h, and problems of microbial contamination The assay requires the use of pancreatic a-amylase and Amyloglucosidase. Both of tese enzymes can be allergenic to some individuals. These enzymes should be weighed in a fume cupboard, and a method is give for preparation of ammonium sulphate suspensions of the enzymes to reduce exposure of the analyst to these components in regular use. Acids and bases: The method requires the use of small quantities of strong acids and bases. These should be handled with proper safety equipment including protective laboratory glasses and gloves. have been removed. Allergenic Enzymes:

AOAC Research Institute ERP Use Only