AOAC RI ERP E-Book - DS DF

OMA 2014.10 C: Collaborative Study Manuscript Expert Review Panel Use Only September, 2017

2013 Dietary Starch in Animal Feeds Collab Study Protocol 072513

Apparatus (a) Grinding mill . – Mills such as abrasion mill equipped with a 1.0 to 0.5 mm screen, or a cutting mill with 0.5 mm screen to grind test samples to pass a 40 mesh screen. (b) Homogenizer, blender or mixer –To provide homogenous suspension of canned pet food, liquid animal feed, semi-moist pet food and other materials containing less than 85% dry matter. (c) Bench centrifuge or microcentrifuge. – Capable of centrifuging at 1000 x g to 10,000 x g . (d) Water bath. – Capable of maintaining 50°C + 1°C. (e) Vortex mixer. (f) pH meter. (g) Stop clock timer (digital). (h) Top-loading balance . – Capable of weighing accurately to + 0.01 g. (i) Analytical balance. – Capable of weighing accurately to + 0.0001 g. (j) Laboratory ovens. - With forced-convection; capable of maintaining 100 + 1°C for carrying out incubations. (k) Spectrophotometer . – Capable of operating at absorbances of 505 nm. (l) Pipettes. – Capable of delivering 0.1, and 1.0 mL; with disposable tips. (m) Positive-displacement repeating pipette. – Capable of accurately delivering 0.1, 1.0, and 3.0 mL. (n) Dispenser. – 1000 mL or greater capacity capable of accurately delivering 20 and 30 mL. (o) Glass test tubes – 16 x 100 mm. (p) 25 x 150 mm Glass tubes with polytetrafluoroethylene ( PTFE)- lined screw caps (approximate volume 55mL) or comparable tubes to hold 51.1 mL and allow for adequate mixing when sealed. (q) Plastic film, or similarly nonreactive material. (r) Magnetic stir plate. (s) Hardened filter paper with 22 µ m retention. Acetic Acid – Flammable liquid and vapor. Causes severe skin burns and eye damage. Toxic if inhaled. Avoid breathing fumes. Wear protective gloves, clothing, and eye and face protection. Alpha-amylase, glucose oxidase - Respiratory sensitizer, may cause allergy or asthma symptoms. Avoid breathing dust. Benzoic acid – Causes serious eye damage, respiratory irritation. Avoid breathing dust and mist. Wear eye protection. AOAC Research Institute ERP Use Only Reagents and Solutions Safety Precautions

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