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OMA 2014.10 C: Collaborative Study Manuscript Expert Review Panel Use Only September, 2017

2013 Dietary Starch in Animal Feeds Collab Study Protocol 072513

of 98.67 + 0.69% for summation of volumes; with omission of the one value, the dilution by weight and summation of volumes still did not differ ( P = 0.64). Based on these results, summation of added volumes or working with dilution by weight are both adequate for determining final sample solution volume. The summation of added volumes is a more rapid approach requiring less equipment and we recommend this approach. Modification: Use of 200 instead of 100 U of amyloglucosidase Use of 200 vs 100 U of amyloglucosidase per sample was evaluated on purified substrates (glucose, corn starch) and feeds (dry ground corn, high moisture shelled corn, 4 corn silage / corn plant samples) that had been dried at 55°C and ground to pass the 1 mm screen of a cyclone mill. Increasing the number of units of amyloglucosidase significantly increased the starch recovery ( P =0.04, Table 3). Because of the improvement in recovery, use of 200 U of amyloglucosidase is recommended. References ( 1) Hall, M. B. (2009) J. AOAC Int. 92 , 42-49 (2) McCleary, B.V., Gibson, T.S., & Mugford, D.C. (1997) J. AOAC Int. 80 , 571-579 (3) AOAC Guidelines for Single Laboratory Validation of Chemical Methods for Dietary Supplements and Botanicals, 2002-12-19 (4) Trinder, P. (1969) J. Clin. Pathol. 22 , 158-161 (5) Karkalas, J. (1985) J. Sci. Food Agric. 36 , 1019-1027 (6) BRENDA, The comprehensive enzyme information system Release 2008.2, Braunschweig Dept. of Bioinformatics, http://www.brenda-enzymes.org (7) Official Methods of Analysis (2005) 18th Ed., AOAC INTERNATIONAL, Gaithersburg, MD, Method 996.11 (8) Hall, M.B., Jennings, J.P, Lewis, B.A., & Robertson, J.B. (2000) J. Sci. Food Agric. 81 , 17-21 (9) Wu, X., Beecher, G. R., Holden, J. M., Haytowitz, D. B., Gebhardt, S. E., & Prior, R. L. (2004) J. Agric. Food Chem. 52 , 4026-4037 (10) Thiex, N, Lawrence Novotny, Charles Ramsey, George Latimer, Laszlo Torma, and Robert Beine. (2000) Guidelines for Preparing Laboratory Samples . Oxford, Association of American Feed Control Officials, Inc. AOAC Research Institute ERP Use Only

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