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OMA 2014.10 C: Collaborative Study Manuscript Expert Review Panel Use Only September, 2017

2013 Dietary Starch in Animal Feeds Collab Study Protocol 072513

Tables 1, 2, and 4 are from JAOACI Volume 92:43, 45, and 47. “Acetate buffer” method is the recommended method for the collaborative study. Tables 5 through 8 are modified from JAOACI Volume 92: 53, 55, 56 and 58 to reflect only values for the glucose oxidase-peroxidase method recommended for the collaborative study. Table 3 contains information evaluated subsequent to the JAOACI publications and relate to slight modifications included in the proposed method. Table 1. Results of determinations for free glucose and starch + maltooligosaccharides corrected for free glucose for purified substrates (dry matter basis). Hot water Acetate buffer Extension of AOAC 996.11

Sample

DM a , % Mean

s r b

Mean

s r b

Mean

s r b

Free glucose Glucose

100.0 100.0 91.8 89.3 90.0

91.8

1.78

90.8 0.17 2.17 0.04 0.04

0.32 0.00 0.01 0.00 0.01

86.0 0.17 1.65 0.06 0.08

0.16 0.00 0.04 0.04 0.01

Sucrose Dextrin

0.10 0.01 2.27 0.04 0.04 0.01 0.02 0.01

Corn starch Potato starch

Starch analysis values Glucose 100.0

90.4

0.3

90.8

0.3

86.0

0.2

Starch + maltooligosaccharides corrected for free glucose c Sucrose 100.0 0.3 1.9 0.7 0.0

1.4 49.8 93.4 94.8

0.0 0.3 1.1 1.3

Dextrin

91.8 89.3 90.0

46.9 93.9 91.2

0.2 1.9 0.3

50.0 98.3 97.0

0.1 0.3 0.3

Corn starch Potato starch

Average for dextrin, corn starch, and potato starch Mean 77.3

81.8 0.22 0.26

79.3 0.88 1.02

s r b

0.82 0.94

CV% d

a DM = dry matter b s r = standard deviation of replicates c Measured values for sucrose, corn starch and potato starch represent starch, not starch +

maltooligosaccharide content, of these substrates. d CV% = coefficient of variation; (s r / mean) x 100 AOAC Research Institute ERP Use Only

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