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OMA 2014.10 C: Collaborative Study Manuscript Expert Review Panel Use Only September, 2017

2013 Dietary Starch in Animal Feeds Collab Study Protocol 072513

Table 5. Historic data and results of the present study on effect of standard solution preparation on form of standard regression curve. Curves represent combined results of 2 replicate assay runs. Curve Form a Intercept Coefficient for Abs b Coefficient for Abs 2 R 2 RMSE c Quadratic term p - value Sum of Squared Residuals Trinder, 1969 L -5.782 505.536 0.9996 7.03 197.5 Q 0.559 472.30 16.36 1.0000 1.48 <0.01 6.56 Time d Fresh glucose solutions in water, 7 point standard curves 0.1:3.0 Standard solution : GOPOD reagent e 45 min L -4.267 899.68 0.9997 4.743 890.0 45 min Q 0.342 871.47 25.9859 0.9999 3.402 <0.01 451.3 140 min L -1.794 899.60 0.9997 5.067 1027.0 140 min Q 3.338 867.66 29.6173 0.9999 3.342 <0.01 435.6 380 min L -0.318 899.60 0.9998 4.502 810.8 380 min Q 3.028 878.92 19.1439 0.9998 3.802 <0.01 563.8

Benzoic acid solutions, 5 point standard curves 0.1:3.0 Standard solution : GOPOD reagent 1 d L -4.054 903.22

0.9998 0.9999 0.9998 1.0000 0.9999 1.0000

4.851 3.307 5.183 1.972 3.964 1.061

305.9 131.2 349.2 46.7 204.2 13.5

1 d 2 d 2 d 3 d 3 d

Q L Q L Q

0.007 -5.232 0.137 -4.669 -0.405

873.80 900.70 861.88 900.55 869.83

26.6310

<0.01

35.0511

<0.01

27.6968

<0.01

a L = Linear, Q = quadratic b Abs = absorbance c RMSE = root mean squared error d Time from standard solution preparation to analysis and reading of absorbance of samples. e GOPOD = glucose oxidase - peroxidase reagent of Karkalas (5)

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