AOAC RI ERP E-Book - DS DF

at 0.5 mL/min. System must be capable of separating maltose from maltotriose (Figure 2017.xxD). Run time of 60 min to ensure that all materials from the injection are cleared from the column prior to the next injection. Cation and anion exchange guard column (containing de-ionising/ de- salting cartridges).— Cation and anion exchange guard cartridges, H + and CO 2 3- forms respectively. (Bio-Rad Laboratories, Cat. No. 125- 0118, includes one cation and one anion cartridge), with guard column holder (Bio-Rad Labratories, Cat. No. 125-039) to hold the two guard cartridges in series, cation cartridge preceding anion cartridge). http://www.bio-rad.com/en-jp/sku/1250118-micro-guard-de-ashing- refill-cartridges. Guard column (or pre-column).— TSKgel ® PW XL guard column. Filters for water.— polyvinylidene fluoride, pore size 0.45 µm, 47 mm. (bb) Filter apparatus.— To hold 47 mm, 0.45 µm filter, B(aa) to filter larger volumes of water. (cc) Syringes.— 10 mL, disposable, plastic. (dd) Syringes.— Hamilton 100 L, 710SNR syringe. (ee) Rotary evaporator .—Heidolph Laborota 4000 or equivalent. http://www.heidolphna.com (ff) Thermometer .—Capable of measuring to 100°C. (v) (w) (x) Detector.— Refractive index (RI); maintained at 50°C. Data integrator or computer.— For peak area measurement. Filters for disposable syringe.— 0.45 µm membrane, 13 mm or 25 mm. (y) (z) (aa)

C. Reagents AOAC Research Institute ERP Use Only

(a) EtOH 95 % v/v. or Industrial Methylated Spirits (IMS). Industrial Methylated Spirits made up of: EtOH 84.8333 (w%), 85.952 (v%); water 5.6571 (w%), 4.524 (v%); 2-propanol 4.9118 (w%), 5.0000 (v%); methanol 4.5979 (w%), 4.524 (v%). Can be prepared by mixing 5 volumes of 2 propanol with 95 volumes of denatured ethanol formula SDA-3A (100 volumes of 95% EtOH combined with 5 volumes of methanol).

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