AOAC RI ERP E-Book - DS DF

(u)(t) High Performance Liquid chromatograph (HPLC).— With oven to maintain a column temperature of 80°C and a 50 µL injection loop. Column operating conditions are: Temperature, 80°C; mobile phase, distilled water, flow rate, 0.5 mL/min. (v)(u) HPLC columns.— Two TSK-GelTSKgel ® G2500PW XL columns, 30 cm × 7.8 mm, connected in series. Operate at 80°C. Mobile phase: distilled water at 0.5 mL/min. System must be capable of separating maltose from maltotriose (Figure 2017.xxD). Run time of 60 min to ensure that all materials from the injection are cleared from the column prior to the next injection. Laboratories, Cat. No. 125-0118, includes one cation and one anion cartridge), with guard column holder (Bio-Rad Labratories, Cat. No. 125-039) to hold the two guard cartridges in series, cation cartridge preceedingpreceding anion cartridge). http://www.bio-rad.com/en- jp/sku/1250118-micro-guard-de-ashing-refill-cartridges. (x)(w) Guard column (or pre-column).— TSK Gel PWx1TSKgel ® PW XL guard column (TOSOH).. (w)(v) Cation and anion exchange guard column (containing de- ashingionising / de-salting cartridges).— Cation and anion exchange guard cartridges, H + and CO 2 3- forms respectively. (Bio-Rad

(y)(x) Detector.— Refractive index (RI); maintained at 50°C. (z)(y) Data integrator or computer.— For peak area measurement. (aa)(z) Filters for disposable syringe.— 0.45 µm membrane, 13 mm or 25 mm. (bb)(aa) Filters for water.— polyvinylidene fluoride, pore size 0.45 µm, 47 mm. (cc)(bb) Filter apparatus.— To hold 47 mm, 0.45 µm filter, [ B(aa )] ; ) to filter larger volumes of water. (dd)(cc) Syringes.— 10 mL, disposable, plastic. (ee)(dd) Syringes.— Hamilton 100 L, 710SNR syringe. (ff)(ee) Rotary evaporator .—Heidolph Laborota 4000 or equivalent. http://www.heidolphna.com (gg)(ff) Thermometer .—Capable of measuring to 100°C. AOAC Research Institute ERP Use Only

Method updated 2008--08-10 This copy printed 22 August 2017

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