AOAC RI ERP E-Book - DS DF

OMAMAN-38 A: Collaborative Study Manuscript Expert Review Panel Use Only September, 2017

Calculations can be simplified by using an Excel ® based calculator (Supporting Information)

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References

Figures: Figure 2017.xxA. Rapid integrated total dietary fiber assay procedure showing the key steps in the procedure. Figure 2017.xxB. Incubation of samples in Fisherbrand ® incubation bottles in a shaking water bath, showing custom made polypropylene bottle holder. Figure 2017.xxC. 2mag Mixdrive 15 ® submersible magnetic stirrer in custom built bath. With Fisherbrand ® incubation bottles. Figure 2017.xxD. Chromatograms of a mixture of maltodextrins, glucose and glycerol on two TSK gel filtration columns (G2500PWXL) in series. Solvent: distilled water; flow rate: 0.5 mL/min; temperature: 80°C. The arrows show demarcation between DP2 (maltose) and DP 3 (higher maltodextrins). The fraction shown as SDFS denotes the fraction that would be collected as SDFS, however, in this case these are maltodextrins that would be hydrolysed by the PAA/AMG mixture. Figure 2017.xxE. Chromatograms on TSK-Gel G2500PWXL columns of glucose/glycerol mixtures. A mixture of glycerol (100 mg) and glucose (100 mg) was analysed according to the RINTDF procedure. The ethanolic filtrate (for SDFS determination) was concentrated to dryness and re-dissolved in 32 mL of deionised water. A sample of this was analysed by HPLC; a) directly with no desalting and no Bio-Rad ® de-ashing pre-cartridges in place; b) a sample (5 mL) was desalted by mixing with 1.5 g of Amberlite ® FPA53 (OH − ) and 1.5 g of Ambersep ® 200 (H + ) resins over 5 min and the supernatant was analysed by HPLC with no Bio-Rad ® de-ashing pre-cartridges in place; c) Sample b) was analysed with a Bio-Rad ® de-ashing pre-cartridges in place. Desalting with resins in a polypropylene tube, as described here, removes > 95% of the salt from the sample, thus ensuring more efficient use of the expensive Bio-Rad ® de-ashing pre-cartridges. This desalting step increases the effectiveness of the de-ashing cartridges and allows up to 10-times more samples to be chromatographed before the need to regenerate or replace the de-ashing cartridges. Figure 2017.xxF. Desalting of samples for HPLC. Five (5) mL of concentrated eluate mixed with 1.5 g of Amberlite ® FPA53 (OH − ) and 1.5 g of Ambersep ® 200 (H + ) resins in a polypropylene tube. AOAC R search Institute ERP Use Only

Method updated 2008--08-10 This copy printed 22 August 2017

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