AOAC RI ERP E-Book - DS DF

12 th May, 2017 AOAC International /AACC International / ICC Interlaboratory Evaluation of A Rapid Integrated Total Dietary Fiber Method consistent with the CODEX Definition of Dietary Fiber AOAC Official Method 2017.xx Total Dietary Fiber in Foods Enzymatic-Gravimetric-High Pressure Liquid Chromatography Method General approach: This study was coordinated by Dr. B. McCleary, Megazyme, and was performed in 2016 under the auspices of AACC International and ICC (International Association of Cereal Science and Technology). The study was performed in three stages; In the First Stage , various laboratories were invited to participate. Those who accepted were: 1 Megazyme, Bray, County Wicklow, Ireland. 2 Medallion Laboratories/General Mills, Golden Valley, MN, USA. 3 Agriculture and Agri-Food Canada/Agriculture et Agroalimentaires Canada, University of Manitoba – Winnipeg, Manitoba, Canada. 4 Grain Growers Limited, PO Box 7, North Ryde, NSW, Australia. 5 Sanitarium Development and Innovation Analytical Department, Cooranbong, NSW, Australia. 6 CRDS Tienen, Central Department Research, Development and Services, Tienen, Belgium. 7 Finnish Food Safety Authority Evira, Chemistry and Toxicology Research Unit, Helsinki, Finland. 8 Matsutani Chemical Company, Itami City, Hyogo, Japan. 9 NEOTRON SPA, Stradello Aggazzotti, Modena, Italy. 10 Eurofins Food Testing Netherlands BV, Heerenveen, Netherlands. 11 Kellogg Company, Battle Creek, MI, U.S.A. 12 Japanese Food Research Laboratories, Japan 13 Nestle, Food Science and Technology Carbohydrates, Nestlé Research Centre Lausanne, Switzerland. In the second stage , four samples were sent to each collaborator along with the assay protocol, all enzymes and reagents required to perform the assay. There were no blind duplicates. The collaborators were requested to analyse the samples for HMWDF and SDFS in duplicate and to report back on any particular difficulties experienced so that these could be addressed before the full collaborative study was initiated. Results were received and compared. The only complication was that two laboratories did not have the

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