AOAC RI ERP E-Book - DS DF

OMAMAN-38 B: Collaborative Study Protocol Expert Review Panel Use Only September, 2017

Determination of Total Dietary Fiber (CODEX Definition) by a Rapid Enzymatic- Gravimetric Method and Liquid Chromatography: Collaborative Study Barry V. McCleary, Jodi Cox, Vincent A. McKie Megazyme, Bray, County Wicklow, Ireland. David Plank Medallion Laboratories/General Mills, Golden Valley, MN, USA. Nancy Ames Agriculture and Agri-Food Canada/Agriculture et Agroalimentaires Canada, University of Manitoba – Winnipeg, Manitoba, Canada. Hayfa Salman Grain Growers Limited, PO Box 7, North Ryde, NSW, Australia. Susan McCarthy Sanitarium Development and Innovation Analytical Department, Cooranbong, NSW, Australia. Saskia Iilians CRDS Tienen, Central Department Research, Development and Services, Tienen, Belgium. Helena Pastell Finnish Food Safety Authority Evira, Chemistry and Toxicology Research Unit, Helsinki, Finland. Toyohide Nishibata Matsutani Chemical Company, Itami City, Hyogo, Japan. Alessandro Santi NEOTRON SPA, Stradello Aggazzotti, Modena, Italy. Peter Sanders Eurofins Food Testing Netherlands BV, Heerenveen, Netherlands. Yulai Jin Kellogg Company, Battle Creek, MI, U.S.A. Mikihiko Yoshida Japanese Food Research Laboratories, Japan. Delphine Curti Nestle, Food Science and Technology Carbohydrates, Nestlé Research Centre Lausanne, Switzerland.

ABSTRACT A rapid, integrated method for measurement of total dietary fiber (TDF) as defined by the Codex Alimentarius Commission was validated for foods. The method is applicable to plant materials, foods, and food ingredients consistent with the 2009 Codex definition (ALINORM 09/32/REP) and it measures insoluble dietary fiber (IDF) and soluble dietary fiber (SDFP plus SDFS). It is an update of AOAC 2009.1, and addresses all of the issues identified by analysts in using that method over the past 8 years. Incubation time with pancreatic α -amylase (PAA) plus amyloglucosidase (AMG) was reduced from 16 h to 4 h, inline with the likely time of residence of food in the small intestine. To ensure complete hydrolysis of non-resistant starch, PAA AOAC Research Institute ERP Use Only

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