September 2016 SPDS Book

Significance

► Most widely used methods for protein determination are combustion and kjeldahl which measure only nitrogen PROTEIN ADULTERATION ► Generally, amino acids are cheaper than high quality protein ingredients ► Amino acids and Taurine can be added to products to “fool” methods and increase the perceived protein content of product ► Common adulterants

 Taurine  Glycine  Alanine

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Analytical Needs

► Method should

 Identify and quantify each relevant proteinaceous amino acid in raw materials and a variety of dietary supplements in which amino acids are a dietary ingredient  Identify and quantify proteinaceous amino acid adulterants in relevant dietary supplement finished products

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