Basic Training 4th Annual ICP Conference

Iodine in biologicals

2 – Sample Preparation Possible approaches

Advantages

Disadvantages

1. Acid digestion 2. Base digestion 3. Ashing/fusion 4. Dilute-and-shoot

minimal equipment, few reagents

few, if any

simple

none

Possible concerns ! Iodide

Method 1. Dilute sample in 1% TMAH* + 0.05% Triton-X 2. Ultrasonicate, dilute in water (if desired) * tetramethylammonium hydroxide

Iodine

Iodine in biologicals

3 – Sample Measurement Optical Emission or Mass Spec?

ICP-OES

ICP-MS

1. Detection limits

dilution factor vs. IDLs

2. Matrix (simple) 3. Wavelengths / Masses 4. Standard compatibility

good options

monoisotopic, few interferences

N/A

N/A

Dilution factors – can be low (5-20x), as sample mostly water, C, N