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AOAC First Action Method 2016.02 (Biotin) MLT Report
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Dilution
= 10 (100 x 1 ÷ 10) Sample made up to 100mL, 10mL used for IAC clean-up to a final volume of
1mL with water for HPLC analysis. The dilution will be 20 if 5mL is used for IAC clean-up.
Slope
= Valid slope calculation based on concentration on X-axis and peak area on Y-axis
Sample weight
: Calculate powder equivalent in grams using the following equation for reconstituted
powder samples.
Sample weight (powder equivalent) in grams = (W1 x W3) / W2
For ready to feed liquid samples, the sample weight used for extraction is used for the calculation.
Report results to two decimal places as biotin or biocytin in
µ
g/100g
3.3.13. Chromatograms
Chromatograms of calibration standard and a SPIFAN MLT sample (00859RF00 / PZGP859) are given
below in Figure 1 and 2 respectively.
Figure 1: Calibration standard
2016.02 (FEBRUARY 2017) BIO-02 MLT REPORT
FOR ERP USE ONLY
DO NOT DISTRIBUTE