34 / 123
AOAC First Action Method 2016.02 (Biotin) MLT Report
Page 10 of 16
Figure 2: MLT Sample 8B (00859RF00 / PZGP859)
4. RESULTS AND DISCUSSION
4.1. The overview
The details of the 12 laboratories from 10 different countries participated in the MLT study is given in Table
2. The laboratories were requested to analyse biotin and biocytin in the samples, however none of the
samples were found to have biocytin and only biotin results were reported and evaluated in the study. All
the biotin results presented in the report are expressed as
µg/100g
of sample as received basis from AOAC
International.
Three of the eleven laboratories (Labs 10, 11 and 12) could only report the results of practice samples on
the due date of the study, 27 Jan 2017. In general, the results from Lab 7 is showing low bias which was
investigated further. The possible reasons for the low bias could be the practice followed in sample
preparation especially the steps related to loading of immunoaffinity column and the elution technique
where triple backflush is specified. The investigation from Lab 7 reported that the analysts did not follow the
backflush technique effectively and that may have caused the incomplete elution of biotin from the
cartridges. The laboratory also reported that they had deviated from the protocol in reconstitution of powder
samples by taking 10g of powder in 80mL. The results from Labs 7, 10, 11 and 12 are included in the MLT
report and for statistical evaluation of the results to calculate precision of the analytical method.
2016.02 (FEBRUARY 2017) BIO-02 MLT REPORT
FOR ERP USE ONLY
DO NOT DISTRIBUTE