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AOAC Final Action Method 2016.02 (Biotin)
Page 12 of 16
Table 2016.02G: Statistical evaluation biotin results from SPIFAN matrices
Samples
Product Description
p
Mean %RSD
r
%RSD
R
HorRat
Practice 1
Infant Formula Powder Partially Hydrolysed Soy Based
12
38.59
3.18
6.98
0.38
Practice 2
Infant Formula Powder FOS/GOS Based
12
14.94
4.92
7.74
0.36
MLT - 1
Infant Formula Powder Partially Hydrolysed Milk Based
9
34.01
5.53
9.48
0.50
MLT - 2
Infant Elemental Powder
9
79.20
2.64
5.14
0.31
MLT - 3
Infant Formula RTF Milk Based
9
4.07
5.52
7.59
0.29
MLT - 4
Adult Nutritional RTF High Fat
9
71.46
3.88
7.75
0.46
MLT - 5
Infant Formula Powder Milk Based
9
26.97
6.79
8.76
0.45
MLT - 6
Infant Formula Powder Soy Based
9
44.75
5.77
6.96
0.39
MLT - 7
NIST SRM 1849a
9
196.5
2.38
3.04
0.21
MLT - 8
Adult Nutritional Powder Low Fat
9
258.7
2.81
7.03
0.51
MLT - 9
Child Formula Powder
9
166.8
7.03
7.03
0.47
MLT - 10
Toddler Formula Powder Milk-Based
9
10.12
5.74
6.65
0.29
MLT - 11
Infant Formula Powder Milk Based
9
42.90
2.26
5.72
0.31
MLT - 12
Adult Nutritional RTF High Protein
9
52.68
5.14
5.89
0.33
p = total number of laboratories
RSD
r
= relative standard deviation of repeatability
RSD
R
= relative standard deviation of reproducibility
HorRat = Horwitz Ratio (RSD
R
/ PRSD
R
)
Table 2016.02H: Comparison with LCMS/MS technique
Sample # Product Description
Blind
Duplicates
Biotin (µg/100g)
AOAC 2016.02
LCMS/MS
Difference
MLT 1
Infant Formula Powder Partially
Hydrolysed Milk Based
A
33.78
35.55
-1.77
B
34.23
39.10
-4.87
MLT 2
Infant Elemental Powder
A
78.79
82.45
-3.66
B
79.61
81.15
-1.54
MLT 3
Infant Formula RTF Milk Based
A
4.02
3.73
0.29
B
4.13
3.84
0.29
MLT 4
Adult Nutritional RTF High Fat
A
71.90
66.06
5.84
B
71.02
77.49
-6.47
MLT 5
Infant Formula Powder Milk Based
A
26.67
26.99
-0.32
B
27.27
25.25
2.02
MLT 6
Infant Formula Powder Soy Based
A
44.36
43.00
1.36
B
45.14
42.27
2.87
MLT 7
NIST SRM 1849a
A
196.06
186.58
9.48
B
196.96
181.50
15.46
MLT 8
Adult Nutritional Powder Low Fat
A
260.88
263.90
-3.02
B
256.49
246.17
10.32
MLT 9
Child Formula Powder
A
174.60
171.69
2.91
B
159.09
162.77
-3.68
MLT 10
Toddler Formula Powder Milk-Based
A
10.38
9.87
0.51
B
9.85
10.30
-0.45
MLT 11
Infant Formula Powder Milk Based
A
42.84
41.66
1.18
B
42.96
39.77
3.19
MLT 12
Adult Nutritional RTF High Protein
A
52.63
54.05
-1.42
B
52.73
51.57
1.16
2016.02 (BIOTIN) FINAL ACTION REVIEW
FOR ERP USE ONLY
DO NOT DISTRIBUTE