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AOAC INTERNATIONAL

Stakeholder Panel on

Infant Formula and Adult Nutritionals

(2016.02 - Biotin Comments)

Method

Method Title

Comments

OMA#

2016.02

AOAC 2016.02:

Determination of biotin by liquid

chromatography coupled with immunoaffinity column

clean-up extraction

o

See reviewer form(s)

o

.12 in the sample with back to back flushing;

information not clear in the original, but

changed in the current version

o

Method was easily applied

o

In the cartridge (address in final version)

o

Method contains unknown content

o

Clarity around the column usage

o

Stability of the column

o

System suitability

o

Ability to use other IA columns

Any immunoaffinity column could be used

(data can be found in the SLV report)