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Summary
A multi-laboratory collaborative study of AOAC First Action 2016.05 (1), an LC-MS/MS method for the
analysis of vitamin D
2
(ergocalciferol) and vitamin D
3
(cholecalciferol) in infant formula and nutritional
products was undertaken. This study consisted of analyses of blind duplicates of 5 different unfortified
nutritional products containing endogenous levels <1 µg hg
-1
, blind duplicates of 13 vitamin D
3
supplemented powders and ready-to-feed nutritional products containing ~1–12 µg hg
-1
and two reference
materials, NIST 1849a and NIST 1869 containing ~11 µg hg
-1
vitamin D
3
and ~14 µg hg
-1
vitamin D
2
respectively by 9 different laboratories. For all supplemented nutritional products, precision as repeatability
ranged from 1.9–5.8% RSD
r
and reproducibility values ranged from 6.4–12.7% RSD
R
, within limits set in the
SPIFAN Vitamin D SMPR (2). Acceptable repeatability and reproducibility was also demonstrated with
HorRat values for the method ranging from 0.2–0.6. On the basis of the results of this study, it is
recommended that AOAC First Action 2016.05 be endorsed as Final Action for the determination of
vitamin D in infant formula and nutritional products.
2016.05 (FEBRUARY 2017) VITD-18
MLT REPORT
FOR ERP USE ONLY
DO NOT DISTRIBUTE