35 / 68
33
SSC (Table 3). However, this characteristic
was associated with the mean fruit weight in
the ’G96-01’ ×
C. kinokuni
‘Mukaku kishu’
PI539530 segregating population, with fruit
from genotypes with enlarged neck having
an average weight of 90.7g in comparison
with their flush counterparts of 137.9g. Sim-
ilarly, fruit from genotypes with a neck had a
SSC of 9.7 in comparison with fruit with no
neck with an average SSC of 10.2. For all the
segregating populations SSC was not corre-
lated with fruit weight.
Conclusions
In the pursuit of developing a seedless cit-
rus cultivar with a heritable seedless trait, it
is important to identify and understand any
affects that this trait may have on fruit size
and sugar content. The research presented
shows that the four families of F1 breed-
ing populations segregating for the seedless
(
Fsfs
) trait, have no significant difference in
fruit weight (g) or SSC (%) from their seeded
counterparts. In addition, the presence of
a neck at the stem segregated in a 3:1 (+/-)
fashion among these populations. This trait
had no consistent effect on the measured pa-
rameters in this study.
Literature Cited
Chao, C.C.T. 2005. Pollination study of mandarins
and the effect on seediness and fruit size: impli-
cations for seedless mandarin production. Hort-
Science. 40:362-365.
Chavez, D. J. and J.X. Chaparro. 2011. Identification of
Markers Linked to Seedlessness in Citrus kinokuni
hort. ex Tanaka and Its Progeny Using Bulked Seg-
regant Analysis. HortScience. 46:693-697.
M
andarin
Table 3.
Fruit weight and soluble solids concentration of Robinson OP ‘GS’ × C. kinokuni segregating population
for genetic seedlessness Fs as separated by presence or absence of fruit neck.
Phenotype
Genotypes (no)
Fruit (no)
Weight (g)
SSC (%)
Neck
138
396
100.31 a
z
8.95 a
Flush (no neck)
40
108
100.70 a
9.05 a
z
Similar letters within a column indicates means not significantly different, Tukey’s test, α=0.05.
Denney, J. O. and G.C. Martin. 1990. Xenia and
metaxenia: definitions and other boundaries. Hort-
Science. 25:1169-1169.
Futch, S. H. and L.K. Jackson. 1993. Pollination of
Citrus Hybrids. UF/IFAS. EDIS Doc.#HS182.
García-Martínez, J. L. and M.A. García-Papí. 1979.
The influence of gibberellic acid, 2, 4-dichloro-
phenoxyacetic acid and 6-benzylaminopurine
on fruit-set of Clementine mandarin. Scientia
Hort. 10:285-293.
García-Papí, M.A., and J.L. García-Martínez. 1984.
Endogenous plant growth substances content
in young fruits of seeded and seedless Clemen-
tine mandarin as related to fruit set and develop-
ment. Scientia Hort. 22:265-274.
Hearn, C. J., P.C. Reece, and R. Fenton. 1968. Effects
of pollen source on fruit characteristics and set
of four citrus hybrids. Proc. Fla. State Hort. Soc.
81:94-98.
Khan, I. A. (Ed.) 2007. Citrus genetics, breeding and
biotechnology. CABI. pp. 198-205.
Nesumi, H., M. Nakano, and T. Yoshida. 2001 Mode
of inheritance on the abnormal development of
impregnated ovules derived from Mukaku-kishu.
J. Jpn. Soc. Hor. Sci. 70 (Suppl. 2):403 (abstr) [in
Japanese].
Spiegel-Roy, P. and E.E. Goldschmidt. 1996. The biol-
ogy of Citrus. Cambridge Univ. Press. pp. 41.
Vithanage, V. 1991. Effect of different pollen parents
on seediness and quality of ‘Ellendale’tangor. Sci-
entia Hort. 48:253-260.
Wallace, H. M. and L.S. Lee. 1999. Pollen source, fruit
set and xenia in mandarins. J. Hort. Sci. Biotech-
nol. 74: 82-86.
Wallace, H. M., B.J. King, and L.S. Lee. 2002. Pol-
len flow and the effect on fruit size in an 'Imperial'
mandarin orchard. HortScience 37:84-86.
Yamasaki, A., A. Kitajima, N. Ohara, M. Tanaka, K.
Hasegawa. 2009. Characteristics of arrested seeds
in Mukaku Kishu-type seedless citrus. J. Jpn. Soc.
Hor. Sci. 78:61-67.