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Abbott Nutrition Division
Simultaneous Determination of Total Vitamin B
6,
B
2,
B
3
and B
1
in Infant
Formula Products by LC-MS/MS Using Enzymatic Digestion
e)
Stable-Isotope Labeled Compounds - Internal Standard Stock Solutions
i)
2
H
3
- Pyridoxal Stock Solution (Approximate Concentration: 70
m
g/mL)
Weigh 7.00 mg (
±
0.1 mg) into a tarred weighing vessel. Quantitatively transfer to a 100-mL
volumetric flask with laboratory water and QS with laboratory water. Mix well and transfer
to a 100-mL amber bottle and Store refrigerated (2˚-8˚C).
Expiration: Until exhausted or evidence of contamination.
ii)
13
C
4
-Pyridoxine Stock Solution (Approximate Concentration: 70
m
g/mL)
Weigh 7.00 mg (
±
0.1 mg) into a tarred weighing vessel. Quantitatively transfer to a 100-mL
volumetric flask with laboratory water and QS with laboratory water. Mix well and transfer
to a 100-mL amber bottle and Store refrigerated (2˚-8˚C).
Expiration: Until exhausted or evidence of contamination.
iii)
3
H
3
-Pyridoxamine Stock Solution (Approximate Concentration: 70
m
g/mL)
Weigh 9.00 mg (
±
0.1 mg) into a tarred weighing vessel. Quantitatively transfer to a 100-mL
volumetric flask with laboratory water and QS with laboratory water. Mix well and transfer
to a 100-mL amber bottle and Store refrigerated (2˚-8˚C).
Expiration: Until exhausted or evidence of contamination.
iv)
13
C
3
-Thiamine Chloride Stock Solution (Approximate Concentration: 100
m
g/mL)
NOTE: The target concentration in sample preparations and working standard
solutions is 22 ng/mL.
Weigh 5.00 mg (
±
0.1 mg) into a tarred weighing vessel. Quantitatively transfer to a 50-mL
volumetric flask with 0.12 N HCl and QS with 0.12 N HCl. Mix well and transfer to a 100-
mL amber bottle and Store refrigerated (2˚-8˚C).
Expiration: Until exhausted or evidence of contamination.
v)
13
C
4
,
15
N
2
-Riboflavin Stock Solution (Approximate Concentration: 73
m
g/mL)
NOTE: The target concentration in sample preparations and working standard
solutions is 8.2 ng/mL.
Weigh 7.3 mg (
±
0.1 mg) into a tarred weighing vessel. Quantitatively transfer to a 100-mL
volumetric flask with 0.1% Acetic Acid and QS with 0.1% Acetic Acid. Mix well and
transfer to a 100-mL amber bottle and Store refrigerated (2˚-8˚C).
Expiration: Until exhausted or evidence of contamination.
vi)
2
H
4
-Niacin Stock Solution (Approximate Concentration: 560
m
g/mL)
33
BVit-02
FOR ERP USE ONLY
DO NOT DISTRIBUTE