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Abbott Nutrition Division

Simultaneous Determination of Total Vitamin B

6,

B

2,

B

3

and B

1

in Infant

Formula Products by LC-MS/MS Using Enzymatic Digestion

e)

Stable-Isotope Labeled Compounds - Internal Standard Stock Solutions

i)

2

H

3

- Pyridoxal Stock Solution (Approximate Concentration: 70

m

g/mL)

Weigh 7.00 mg (

±

0.1 mg) into a tarred weighing vessel. Quantitatively transfer to a 100-mL

volumetric flask with laboratory water and QS with laboratory water. Mix well and transfer

to a 100-mL amber bottle and Store refrigerated (2˚-8˚C).

Expiration: Until exhausted or evidence of contamination.

ii)

13

C

4

-Pyridoxine Stock Solution (Approximate Concentration: 70

m

g/mL)

Weigh 7.00 mg (

±

0.1 mg) into a tarred weighing vessel. Quantitatively transfer to a 100-mL

volumetric flask with laboratory water and QS with laboratory water. Mix well and transfer

to a 100-mL amber bottle and Store refrigerated (2˚-8˚C).

Expiration: Until exhausted or evidence of contamination.

iii)

3

H

3

-Pyridoxamine Stock Solution (Approximate Concentration: 70

m

g/mL)

Weigh 9.00 mg (

±

0.1 mg) into a tarred weighing vessel. Quantitatively transfer to a 100-mL

volumetric flask with laboratory water and QS with laboratory water. Mix well and transfer

to a 100-mL amber bottle and Store refrigerated (2˚-8˚C).

Expiration: Until exhausted or evidence of contamination.

iv)

13

C

3

-Thiamine Chloride Stock Solution (Approximate Concentration: 100

m

g/mL)

NOTE: The target concentration in sample preparations and working standard

solutions is 22 ng/mL.

Weigh 5.00 mg (

±

0.1 mg) into a tarred weighing vessel. Quantitatively transfer to a 50-mL

volumetric flask with 0.12 N HCl and QS with 0.12 N HCl. Mix well and transfer to a 100-

mL amber bottle and Store refrigerated (2˚-8˚C).

Expiration: Until exhausted or evidence of contamination.

v)

13

C

4

,

15

N

2

-Riboflavin Stock Solution (Approximate Concentration: 73

m

g/mL)

NOTE: The target concentration in sample preparations and working standard

solutions is 8.2 ng/mL.

Weigh 7.3 mg (

±

0.1 mg) into a tarred weighing vessel. Quantitatively transfer to a 100-mL

volumetric flask with 0.1% Acetic Acid and QS with 0.1% Acetic Acid. Mix well and

transfer to a 100-mL amber bottle and Store refrigerated (2˚-8˚C).

Expiration: Until exhausted or evidence of contamination.

vi)

2

H

4

-Niacin Stock Solution (Approximate Concentration: 560

m

g/mL)

33

BVit-02

FOR ERP USE ONLY

DO NOT DISTRIBUTE