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Abbott Nutrition Division

Simultaneous Determination of Total Vitamin B

6,

B

2,

B

3

and B

1

in Infant

Formula Products by LC-MS/MS Using Enzymatic Digestion

b)

Mass Spectrometer instrument configuration and operating parameters are provided below.

Table 2. Mass spectrometer configuration and operation parameters

Instrument

Quattro Premier XE

Ionization Mode

ESI+ (Electrospray; Positive Ion)

Capillary Voltage

3.50

Cone Voltage

Various

Extractor (V)

5.0

RF Lens (V)

0.0

Source Temperature

120 ºC

Desolvation Temperature

325 ºC

Cone Gas Flow

50 L/Hr

Desolvation Gas Flow

800 L/Hr

LM 1 (Low Mass) Resolution

10

LM 2 (Low Mass) Resolution

10

Ion Energy 1

0.2

Entrance Lens

1

Collision Energy

Various

Exit Lens

1

HM 1 (High Mass) Resolution

10

HM 2 (High Mass) Resolution

10

Ion Energy 2

1.0

Multiplier

710

Collision Cell Pressure

~4 e-3

These parameters are guidelines for the operation of the Quattro Premier XE. These

parameters may be changed at the discretion of a qualified LC-MS/MS scientist and the

approval of the method supervisor based on the instrument performance provided

all system

suitability and control sample parameters are met.

28

BVit-02

FOR ERP USE ONLY

DO NOT DISTRIBUTE