Background Image
Table of Contents Table of Contents
Previous Page  262 / 620 Next Page
Information
Show Menu
Previous Page 262 / 620 Next Page
Page Background

Abbott Nutrition Division

Simultaneous Determination of Total Vitamin B

6,

B

2,

B

3

and B

1

in Infant

Formula Products by LC-MS/MS Using Enzymatic Digestion

c)

Bottle-Top Dispenser: Capable of dispensing volumes of 25.0mL (

±

1mL).

Example: Brand Tech Dispensette III, or equivalent.

d)

Vortex Mixer: Vortex-Genie, or equivalent.

e)

Laboratory Refrigerator

f)

Laboratory Freezer

g)

Magnetic Stir Plate: Variomag multipoint electronic magnetic stir-plate, or equivalent.

h)

Room Light Shields: A.L.P. Protect-A-Lamp, UV cutoff at 460 nm.

i)

Volumetric Flasks: Various sizes including 5-mL, 10-mL 25-mL, 50-mL, 100-mL, 250-mL, 500-

mL, 1000-mL and 2000-mL.

j)

Disposable Pasteur Pipettes

k)

Amber Bottles: Volume capacity of 50 or 100 mL.

l)

Self-Standing Polypropylene Tubes: Cryovials; 5-mL and 10-mL volume capacity.

m)

Weighing Vessels: Various including disposable weighing boats and glass weighing funnels.

n)

Positive Displacement Pipettes: Gilson Microman 10, 25, 100, 250, and 1000 µL.

o)

Positive Displacement Pipette Tips: Gilson Capillary Piston 10, 25, 100, 250, and 1000 µL.

p)

Plastic Syringes. B&D 3-mL, or equivalent.

q)

Syringe Filters: PTFE 0.20µm syringe filters, Acrodisc 25-mm, or equivalent.

r)

50-mL Self-Standing Transport Tubes: Superior Scientific, Ltd., or equivalent.

s)

Autosampler Vials: Waters autosampler vials; 9-mm amber with screw top 12x32 mm pre-split

PTFE-silicon septa; Waters Part # 186000847C.

2.

Instrumentation

NOTE: The following instrumentation has been specifically demonstrated as adequate

for the implementation of this method. Comparable instrumentation may be

substituted with sufficient qualification.

a)

Liquid Chromatograph (LC): Waters Acquity Ultra Performance Liquid Chromatograph (UPLC).

b)

Chromatographic Columns: 2.1 x 50 mm, 1.7-µm Waters BEH C-18Column, and 2.1 x 5mm

Vanguard Column.

c)

Prefilter for Waters Acquity UPLC Column.

d)

Waters Acquity UPLC 10µL Sample Loop.

t)

Tamdem Quadrupole Mass Spectrometer (MS/MS) equipped with an Electrospray Ionization

(ESI) Source: Waters Quattro Premier XE or equivalent.

e)

IBM Personal Computer or equivalent. Suitable for operation of the chromatography data system

software specified below.

26

BVit-02

FOR ERP USE ONLY

DO NOT DISTRIBUTE