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Abbott Nutrition Division

Simultaneous Determination of Total Vitamin B

6,

B

2,

B

3

and B

1

in Infant

Formula Products by LC-MS/MS Using Enzymatic Digestion

d)

Riboflavin

Riboflavin is readily soluble in dilute alkaline solutions and is only sparingly soluble in

water although the solubility of Riboflavin increases in the presence of aromatic compounds

including Niacinamide. Crystalline Riboflavin is stable but in solution it is destroyed by UV

radiation and visible light. Aqueous solutions between pH=2 and pH=5 are heat stable whereas

alkaline solutions are unstable. Riboflavin is stable in the presence of oxygen. Total Riboflavin

activity consists of riboflavin, flavin mononucleotide (FMN) and flavin adenine dinucleotide

(FAD) predominately. Aqueous Riboflavin solutions exhibit absorbance maxima at 223 nm, 266

nm, 373 nm and 445 nm.

The molecular formula for Riboflavin is C

17

H

20

N

4

O

6

and its molecular weight is 376.37 g/mol.

Common synonyms include vitamin B

2

and 7,8-dimethyl-10-ribitylisoalloxazine.

The CAS# for Riboflavin is 83-88-5.

The chemical structure of Riboflavin is shown below:

e)

Thiamin Chloride Hydrochloride

Thiamine Chloride Hydrochloride is readily soluble in water, soluble in glycerol, sparingly

soluble in ethanol and insoluble in organic solvents. Thiamine Chloride Hydrochloride powder is

hygroscopic. Dilute acidic solutions of Thiamine Chloride Hydrochloride are relatively stable to

heat but stability decreases as solution-pH increases. Thiamine, thiamine phosphate and thiamine

diphsophate are to the primary components contributing to the total vitamin B1 activity.

The molecular formula for Thiamine Chloride Hydrochloride is C

12

H

18

Cl

2

N

4

OS and its

molecular weight is 336.27 g/mol.

N

N

N

N

H

2

C C C

H

C

OH

H

OH

H

CH

2

OH

OH

H

3

C

H

3

C

O

O

23

BVit-02

FOR ERP USE ONLY

DO NOT DISTRIBUTE