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Abbott Nutrition Division

Simultaneous Determination of Total Vitamin B

6,

B

2,

B

3

and B

1

in Infant

Formula Products by LC-MS/MS Using Enzymatic Digestion

working standards. Target Lynx uses the powder weight, reconstitution weight, sample weight

and final weight of the sample entered into MassLynx to calculate analyte concentration in the

original sample. As a result this calculated value will be significantly greater than the analyte

concentrations in working standard solutions. The response ratio (ratio of peak area of native

compound to internal standard) is not affected by the dilution calculations.

ii)

If the response ratio for a sample is outside of the response ratio for the corresponding

compound calibration curve sample analysis must be repeated using an adjusted sample weight

such that the level of the native compound produces a response ratio that falls within the

calibration range.

44

BVit-02

FOR ERP USE ONLY

DO NOT DISTRIBUTE