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Table 5. Calibration standards and LOQ for

Total B1 Total B2 Total B3 Total B6

Thiamine Riboflavin Nicotinamide Nicotinic acid Pyridoxal

Pyridoxamine Pyridoxine

Vitamin

ng/mL

ng/mL

ng/mL

ng/mL

ng/mL

ng/mL

ng/mL

ng/mL

ng/mL

ng/mL

ng/mL

WS1

0.196

0.173

1.903

0.255

0.196

0.173

1.766

0.137

0.014

0.016

0.225

WS2

0.489

0.434

4.758

0.638

0.489

0.434

4.416

0.342

0.034

0.041

0.563

WS3

0.978

0.867

9.515

1.276

0.978

0.867

8.831

0.684

0.069

0.082

1.125

WS4

1.956

1.734

19.03

2.551

1.956

1.734

17.662

1.368

0.137

0.164

2.250

WS5

4.890

4.335 47.576

6.378

4.890

4.335

44.156

3.420

0.343

0.410

5.625

WS6

9.780

8.671 95.152 12.755

9.780

8.671

88.312

6.840

0.685

0.820

11.250

LOQ*

9.79

8.65

95.2

12.8 ug/100g

SMPR LOQ

≤ 20

≤ 30

≤ 200

≤ 10 ug/100g

* LOQ is calculated by multiplying the lowest calibration standard by the dilution factor (500) and converting for units

BVit-02 - MEF FORM

FOR ERP USE ONLY

DO NOT DISTRIBUTE