68 / 620
t / min
0 (free)
30 60 90 120 150 180 240 300 360
From the data (see appendix 4) it is seen that a saponification time of 240 min is
sufficient to convert all bound choline to the free forms for all the tested matrixes.
Choline:
0
500
1000
1500
2000
2500
3000
Choline (mg/kg)
Time (min)
Saponification 100°C
raw egg
Petfood
Infant formula
Cornflakes
Fishfeed
Carn-06 (Choline Validation Report)
FOR ERP USE ONLY
DO NOT DISTRIBUTE