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HPLC Determination of Total Tryptophan in Infant Formula and

Adult/Pediatric Nutritional Formula Following Enzymatic

Hydrolysis

Abbott Nutrition, Division of Abbott Laboratories

page

7

K. CONTRIBUTING REFERENCES

1. Narahashi, Y.; Yanagita, M. : (1967) J. Biochemistry 6 pp 633-641.

2. Yamaskov, I.A.; Tichonova, T.V.; Davankov, V.A.; (1986) ENZYME MICROB.

TECHNOL. 8 pp241-244.

L. SPECIFICITY - Selectivity for the analyte of interest is supported by the following:

1. The selectivity of chromatographic separation combined with native fluorescence

2. Near 100% recovery of reference material

Note: A modification recommended by the submitting laboratory is that an additional

standard dilution that is below the typical blank peak response.

Amino-02

FOR ERP USE ONLY

DO NOT DISTRIBUTE