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Is food allergen analysis flawed
? (Cont’d)
•
Risk assessment and risk management of food allergens depend on the
ability to detect and accurately quantify them reproducibly.
•
All current analytical methods exhibit deficiencies that jeopardize accurate
results and risk false positives and false negatives.
•
ELISA is widely used for an extensive period of time, and evidence of
deficiencies are published.
•
PCR and LC-MS/MS are newer technologies, similar deficiencies are not
studied but are expected to be similar.
o
Better proteomic and genomic databases / bioinformatics help
•
Uncertainties remain:
9
Clinical vs Bioinformatics
9
Reference material vs calibrants
9
Allergen structural changes by food processing
9
Impact of food matrix
9
Extraction efficiency
9
Quantification depends on markers chosen
9
Diversity of foods and commodities in global market
27
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This document should not be reproduced or disclosed without prior authorisation
Complexity of allergen sampling plan