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CODEX STAN 234-1999

22

Foods for Special Dietary Uses

Infant formula

Folic acid

AOAC 992.05

(Measures free folic acid + free, unbound

natural folates, aggregated and measured as

folic acid)

EN 14131

(Total folate (free + bound), aggregated and

measured as folic acid)

Microbioassay

II

Infant formula

Folic acid

J AOAC Int. 2000:83; 1141-1148

(Measures free folic acid + proportion of free,

natural folate)

Optical Biosensor

Immunoassay

IV

Infant formula

Folic acid

J Chromatogr. A., 928, 77-90, 2001

(Measures total folates after conversion to,

and measurement as 5-Me-H4PteGlu)

HPLC, incorporating immunoaffinity

clean-up and conversion to 5-

methyltetrahydrofolate

IV

Infant formula

Iodine

(for milk-based formula)

AOAC 2012.15 | ISO 20647 | IDF 234

ICP-MS

II

Infant formula

Iron

9

AOAC 985.35

Flame atomic absorption

spectrophotometry

III

Infant formula

Iron

AOAC 984.27

ICP emission spectroscopy

III

Infant formula

Iron

AOAC 999.11│NMKL139

AAS after dry ashing

II

Infant formula

Magnesium

ISO 8070 │ IDF 119

Flame atomic absorption

spectrophotometry

II

Infant formula

Magnesium

AOAC 985.35

Flame atomic absorption

spectroscopy

III

Infant formula

Magnesium

AOAC 984.27

ICP emission spectroscopy

III

Infant formula

Manganese

AOAC 985.35

Flame atomic absorption

spectrophotometry

II

Infant formula

Manganese

AOAC 984.27

ICP emission spectroscopy

III

Infant formula

Melamine

ISO/TS 15495 | IDF/RM 230

LC-MS/MS

IV

9

General Codex methods are also available

68