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Validation of segmentation algorithms

is crucial

1. Histopathology (gold standard)

Difficult to assess.

Changes in volume and orientation may be challenging

2. Validation with phantom measurements

Necessary to have

Real tumours are often not spherical

Cannot substitue validation with pathology

3. Comparison to manual delineations

Intra-observer variations

Regularized auto-segmentation method may always yield

similar accuracy