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774 

B

ird

et al

.

:

J

ournal of

AOAC I

nternational

V

ol

. 98, N

o

. 3, 2015

Table 2. Total yeast and mold log

10

counts for frozen raw ground beef patties by 3M Petrifilm RYM Count Plate after 48 h incubation at 25

°

C versus FDA BAM (ref. 1) and ISO

21527 (ref. 4) reference methods

a

Uninoculated control

Low

Medium

High

Petrifilm RYM FDA BAM/ISO 21527

b

Petrifilm RYM FDA BAM/ISO 21527

Petrifilm RYM

FDA BAM/ISO 21527

Petrifilm RYM FDA BAM/ISO 21527

Lab

A

c

B

A

B

A

B

A

B

A

B

A

B

A

B

A

B

1

<1.00

<1.00

<1.00

<1.00

2.18

1.78

2.23

1.60

3.45

3.38

3.49

3.32

4.64

4.62

4.32

4.54

2

<1.00

<1.00

<1.00

<1.00

2.43

2.48

2.43

2.83

3.62

3.71

3.49

3.45

4.62

4.73

4.53

4.52

3

<1.00

<1.00

<1.00

<1.00

2.34

2.30

2.41

2.32

3.52

3.30

3.63

3.53

4.64

4.40

4.82

4.81

4

d

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

5

<1.00

<1.00

<1.00

<1.00

1.95

1.78

1.70

2.00

3.41

3.53

3.30

3.41

4.43

4.51

4.46

4.64

6

d

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

7

<1.00

<1.00

<1.00

<1.00

2.23

2.36

1.80

1.82

3.51

3.56

3.36

3.61

4.79

4.32

4.43

4.49

8

d

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

9

<1.00

<1.00

<1.00

<1.00

2.45

2.23

2.28

2.23

3.56

3.60

3.51

3.45

4.72

4.73

4.57

4.79

10

<1.00

<1.00

<1.00

<1.00

2.20

2.20

2.11

2.00

3.60

3.45

3.45

3.43

4.76

4.61

4.66

4.40

11

<1.00

<1.00

<1.00

<1.00

2.36

1.00

2.40

1.90

3.57

3.66

3.49

3.54

4.68

4.56

4.49

4.59

12

d

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

13

<1.00

<1.00

<1.00

<1.00

2.45

2.08

2.00

1.67

3.49

3.51

3.52

3.48

4.75

4.89

4.67

4.77

14

<1.00

<1.00

<1.00

<1.00

2.20

1.00

2.34

e

1.00

e

3.41

3.67

3.57

3.70

4.70

4.60

4.65

4.62

15

<1.00

<1.00

<1.00

<1.00

2.30

2.28

2.15

2.23

3.38

3.53

3.38

3.23

4.72

4.85

4.61

4.66

a

 Log

10

yeast and mold CFU/g.

b

 FDA BAM and ISO 21257 reference method replicates were enumerated at 5 and 7 days (uninoculated test portions) of incubation at 25

°

C

c

 A and B = Indicated duplicate test portions.

d

 Laboratory data not included in statistical analysis for all contamination levels.

e

 Cochran’s outlier.