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774 Bird et al.: Journal of AOAC International Vol. 98, No. 3, 2015
Table 2. Total yeast and mold log
10
counts for frozen raw ground beef patties by 3M Petrifilm RYM Count Plate after 48 h incubation at 25
°C versus FDA BAM (ref. 1) and ISO
21527 (ref. 4) reference methods
a
Uninoculated control
Low
Medium
High
Petrifilm RYM FDA BAM/ISO 21527
b
Petrifilm RYM FDA BAM/ISO 21527
Petrifilm RYM
FDA BAM/ISO 21527
Petrifilm RYM FDA BAM/ISO 21527
Lab
A
c
B
A
B
A
B
A
B
A
B
A
B
A
B
A
B
1
<1.00
<1.00
<1.00
<1.00
2.18
1.78
2.23
1.60
3.45
3.38
3.49
3.32
4.64
4.62
4.32
4.54
2
<1.00
<1.00
<1.00
<1.00
2.43
2.48
2.43
2.83
3.62
3.71
3.49
3.45
4.62
4.73
4.53
4.52
3
<1.00
<1.00
<1.00
<1.00
2.34
2.30
2.41
2.32
3.52
3.30
3.63
3.53
4.64
4.40
4.82
4.81
4
d
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
5
<1.00
<1.00
<1.00
<1.00
1.95
1.78
1.70
2.00
3.41
3.53
3.30
3.41
4.43
4.51
4.46
4.64
6
d
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
7
<1.00
<1.00
<1.00
<1.00
2.23
2.36
1.80
1.82
3.51
3.56
3.36
3.61
4.79
4.32
4.43
4.49
8
d
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
9
<1.00
<1.00
<1.00
<1.00
2.45
2.23
2.28
2.23
3.56
3.60
3.51
3.45
4.72
4.73
4.57
4.79
10
<1.00
<1.00
<1.00
<1.00
2.20
2.20
2.11
2.00
3.60
3.45
3.45
3.43
4.76
4.61
4.66
4.40
11
<1.00
<1.00
<1.00
<1.00
2.36
1.00
2.40
1.90
3.57
3.66
3.49
3.54
4.68
4.56
4.49
4.59
12
d
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
13
<1.00
<1.00
<1.00
<1.00
2.45
2.08
2.00
1.67
3.49
3.51
3.52
3.48
4.75
4.89
4.67
4.77
14
<1.00
<1.00
<1.00
<1.00
2.20
1.00
2.34
e
1.00
e
3.41
3.67
3.57
3.70
4.70
4.60
4.65
4.62
15
<1.00
<1.00
<1.00
<1.00
2.30
2.28
2.15
2.23
3.38
3.53
3.38
3.23
4.72
4.85
4.61
4.66
aLog
10
yeast and mold CFU/g.
bFDA BAM and ISO 21257 reference method replicates were enumerated at 5 and 7 days (uninoculated test portions) of incubation at 25
°C
cA and B = Indicated duplicate test portions.dLaboratory data not included in statistical analysis for all contamination levels.eCochran’s outlier.