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METHOD FOR THE ESTIMATION OF TOTAL PHENOLIC CONTENT USING THE FOLIN-
C ASSAY
Page
5
of
5
For Raw material:
Range
% Accuracy
80%
102
100%
99
120%
104
For extract:
Range
% Accuracy
80%
98
100%
105
120%
101
10.4.
Repeatability:
Repeatability study of Raw material and Extract were carried out
by preparing and analyzing same sample at three different times. The limit of % RSD
should be in the range of ≤9%.
For Raw material:
Sample
%Phenols
Average
%RSD
OA150152-Trial-1
2.46
2.44
1.85
OA150152- Trial-2
2.47
OA150152- Trial-3
2.39
For Extract:
Sample
%Phenols
Average
%RSD
SREE-00-080202002- Trial -1
22.77
22.40
1.47
SREE-00-080202002- Trial -2
22.16
SREE-00-080202002- Trial -3
22.26
Conclusion:
The %RSD is in the range of 1.78 & 1.47 respectively for Raw material &
extract.
10.5.
Stability of the extract:
Stability study of the Salacia extract was conducted
both at real time (25° C±2° C and 60% RH±5% RH) and accelerated (40° C±2° C and
75% RH±5% RH). The extract is found to be stable confirming the ruggedness of the
methodology (Different day & Different analysis).