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Estimetion of Total Phenolic content using the Folin-C assay
Validation Data Of Polyphenol content by Folin Ciocalteu's reagent in Salacia
1)
Analytical Range:
Sr.No.Gallic acid
Concentartion in ppm Absorbance
1
5
0.101
2
25
0.325
3
50
0.651
4
100
1.273
2)
Limit of Quantification:
Weight Of Standard
(mg)
Absorbance of 5ppm
concentration
50.6
0.101
50.2
0.109
51.2
0.118
49.5
0.101
50.4
0.102
50.2
0.098
Average
50.35
0.10
Standard Deviation
0.56
0.01
% RSD
1.11
7.07
3)
Recovery
For Raw material
(B.No-OA150152)
Range (%)
Theoratical Value
Practical Value
% Accuracy
80
1.96
2.00
102.04
100
2.46
2.44
99.19
120
2.95
3.08
104.41
For Extract
(B.No-SREE-00-080202002)
Range (%)
Theoratical Value
Practical Value
% Accuracy
80
17.60
17.23
97.90
100
22.26
23.45
105.35
120
25.90
26.22
101.24
4)
Repeatability
Gallic acid
II Dilution
Weight
50.6
0.5
Dilution
50
10
Purity
99
Absorbance
0.651
Sample Details
Sample wt
Dilution
Absorbance
Result (%)
OA150152-T1
2076.70
50
2
25
1.063
2.46
OA150152-T2
2011.20
50
2
25
1.033
2.47
OA150152-T3
2005.80
50
2
25
0.996
2.39
Average
2.44
SD
0.05
%RSD
1.85
II-Dilution
0.101
0.325
0.651
1.273
y = 0.0124x + 0.0285
R² = 0.9996
0
0.2
0.4
0.6
0.8
1
1.2
1.4
0
20
40
60
80
100
120
Absorabance
Concentartion in ppm
Calibration curve of Gallic acid