ABBOTTNUTRITIONDIVISION
TITLE
PAGENO.
Determination of TransVitaminK
1
byHPLC andFluorescenceDetection
12 of 14
C
c
= slope x peak area+ intercept
C
c
=15,660.01
g/Lx peak area +4,904.21
g/L
Correlation coefficient (r) =0.999939
e) Calculation of TransVitaminK
1
inProduct Samples
Assume a peak areameasurement for a 2.255 gram sample to be 402530.
1) Concentration of injected sample from the regression equation
C
c
=15,660.01µg/Lx 402530+4,904.21µg/L
C
c
=25.39µg/L
2) Concentration of trans vitaminK
1
in the sample
kg g
g
mL
Lg
S
C
C
c
p
/ 6. 112
255 .2
0.10 /
39.25 0.10
VitK-02
FORWORKINGGROUP/ERPUSEONLY
DONOTDISTRIBUTE
